EP 1590460 A1 20051102 - HYPERACTIVE, NON-PHOSPHORYLATED, MUTANT TRANSPOSASES OF MARINER MOBILE GENETIC ELEMENTS
Title (en)
HYPERACTIVE, NON-PHOSPHORYLATED, MUTANT TRANSPOSASES OF MARINER MOBILE GENETIC ELEMENTS
Title (de)
MUTIERTE MARINER TRANPOSASEN ALS MOBILE GENETISCHE ELEMENTE, DIE NICHT PHOSPHORYLIERBAR UND HYPERAKTIV SIND
Title (fr)
TRANSPOSASES D ELEMENTS GENETIQUES MOBILES MARINER MUTANTES, NON PHOSPHORYLABLES ET HYPERACTIVES
Publication
Application
Priority
- FR 2004000161 W 20040123
- FR 0300905 A 20030128
Abstract (en)
[origin: FR2850395A1] A hyperactive transposase mutant (A) from a mariner mobile genetic element (EGM) is new. It has at least one mutation of a phosphorylatable residue (pR), in at least one phosphorylation site, that renders the site non-phosphorylatable. A hyperactive transposase mutant (A) from a mariner mobile genetic element (EGM) is new. It has at least one mutation of a phosphorylatable residue (pR), in at least one phosphorylation site, that renders the site non-phosphorylatable. pR is any of Thr 24, 42, 88, 135, 154, 181, 216 or 255; Ser 28, 104, 147, 170 or 305; or Tyr 171, in a 345 amino acid (aa) sequence (S2), defined in the specification, or at corresponding sites in other mariner EGM, aligned with (S2). Independent claims are also included for the following: (1) recombinant nucleic acid (I) that encodes (A); (2) recombinant vector containing at least one (I); (3) recombinant host cell that harbors at least one vector of (2); and (4) method for producing (A).
IPC 1-7
IPC 8 full level
C12N 9/22 (2006.01); C12N 15/54 (2006.01); C12N 15/55 (2006.01); A61K 38/00 (2006.01)
CPC (source: EP US)
C12N 9/22 (2013.01 - EP US); A61K 38/00 (2013.01 - EP US)
Citation (search report)
See references of WO 2004078981A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
FR 2850395 A1 20040730; FR 2850395 B1 20071214; CA 2514730 A1 20040916; EP 1590460 A1 20051102; JP 2006518220 A 20060810; US 2007031967 A1 20070208; US 7732209 B2 20100608; WO 2004078981 A1 20040916; WO 2004078981 A8 20050909
DOCDB simple family (application)
FR 0300905 A 20030128; CA 2514730 A 20040123; EP 04704632 A 20040123; FR 2004000161 W 20040123; JP 2006505659 A 20040123; US 54371804 A 20040123