EP 1601787 A1 20051207 - DRY REAGENT STRIP AND NUCLEIC ACID DETECTION
Title (en)
DRY REAGENT STRIP AND NUCLEIC ACID DETECTION
Title (de)
TROCKEN-TESTSTREIFEN UN DETEKTION VON NUKLEINSÄURE
Title (fr)
BANDELETTE D'ESSAI A REACTIF SEC ET DETECTION D'ACIDE NUCLEIQUE
Publication
Application
Priority
- GR 0300052 W 20031107
- GR 2002100485 A 20021107
Abstract (en)
[origin: WO2004042084A1] This invention provides a dry reagent test strip having oligonucleotide conjugated gold nanoparticles as an integral part and a method for the preparation of said test strip for the detection and/or determination of a nucleic acid. Oligonucletide conjugated gold nanoparticles are stabilized in dry form and retain all their properties after rehydration (mobility, hybridization capacity and colloidal state). The presence of a nucleic acid in the sample is detected by the formation of two red bands on the test and control zones, as a result of aggregation of gold nanoparticles at the test and control zone by the interaction with binding protein streptavidin and the complementary oligonucleotides, respectively. This invention represents an exceptionally sensitive and fast nucleic acid detection system, easy to use, with no need for special equipment. It has been designed so that to be effective in the detection of any kind of a nucleic acid, the specificity being determined solely by the sequence of the nucleotide- probe-1.
IPC 1-7
IPC 8 full level
CPC (source: EP)
C12Q 1/6837 (2013.01); B01L 3/5023 (2013.01)
C-Set (source: EP)
Citation (search report)
See references of WO 2004042084A1
Citation (third parties)
Third party :
- GR 1003966 B 20020806 - SOUFLA DIMITRIOU GIANNOULA
- MEDICON HELLAS S.A.: "Development of dry reagents for detection of DNA and applications in molecular diagnostics", TECHNICAL BULLETIN, 1999, pages 1 - 26+10 PG-S DRAWINGS, XP003017495
- MIRKIN C.A. ET AL.: "A DNA based method for rationally assembling nanoparticles into macroscopic materials", NATURE, vol. 382, 1996, pages 607 - 609, XP002113276
- ALIVISATORS A.P. ET AL.: "Organization of nanocrystal molecules using DNA", NATURE, vol. 382, 1996, pages 609 - 611, XP002222113
- LETSINGER R.L. ET AL.: "Use of hydrophobic substituents in controlling self-assembly of oligonucleotides", J AM CHEM SOC, vol. 115, 1993, pages 7535 - 7536, XP003017496
- STORHOFF: "One-pot colorimetric differentiation of polynucleotides with single base imperfection using gold nanoparticle probes", J AM CHEM SOC, vol. 120, 1998, pages 1959 - 1964, XP002294465
- MUCIC R.C. ET AL.: "DNA-DIRECTED SYNTHESIS OF BINARY NANOPARTICLE NETWORK", J AM CHEM SOC, vol. 120, 1998, pages 12674 - 12675, XP002936277
- MITCHELL G.P. ET AL.: "Programmed Assembly of DNA Functionalized Quantum Dots", J AM CHEM SOC, vol. 121, 1999, pages 8122 - 8123, XP002143842
- STORHOFF J.J. ET AL.: "PROGRAMMED MATERIALS SYNTHESIS WITH DNA", CHEMICAL REVIEWS, vol. 99, 1999, pages 1849 - 1862, XP002941100
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2004042084 A1 20040521; AU 2003278398 A1 20040607; EP 1601787 A1 20051207; GR 1004393 B 20031128
DOCDB simple family (application)
GR 0300052 W 20031107; AU 2003278398 A 20031107; EP 03769703 A 20031107; GR 2002100485 A 20021107