Global Patent Index - EP 1602730 A3

EP 1602730 A3 20060125 - Biosynthetic production of 4-amino-4-deoxychorismate (ADC) and [3R,4R]-4-amino-3-hydroxycyclohexa-1,5-diene-1-carboxylic acid (3,4-CHA)

Title (en)

Biosynthetic production of 4-amino-4-deoxychorismate (ADC) and [3R,4R]-4-amino-3-hydroxycyclohexa-1,5-diene-1-carboxylic acid (3,4-CHA)

Title (de)

Biosynthetische Herstellung von 4-Amino-4-deoxychorismat (ADC) und [3R,4R]-4-Amino-3-hydroxycyclohexa-1,5-dien-1-carbonsäure (3,4-CHA)

Title (fr)

Préparation biosynthétique de 4-amino-4-deoxychorismate (ADC) et de [3R,4R]-4-amino-3-hydroxycyclohexa-1,5-diene-1-acide carboxylique (3,4-CHA)

Publication

EP 1602730 A3 20060125 (EN)

Application

EP 04076644 A 20040604

Priority

EP 04076644 A 20040604

Abstract (en)

[origin: EP1602730A2] The invention relates to a process for the biosynthetic production of 4-amino-4-deoxychorismate (ADC) performed fermentatively in vivo with a 4-amino-4-deoxychorismate synthase, preferably a PabAB bipartite protein (which may be a fusion protein), at an increased level of activity, thereby obtaining a broth comprising ADC and 4-amino-4-deoxyprephenate (ADP), that are recovered. The invention also relates to a further process of converting the ADP into p-aminophenylalanine. The invention, moreover relates to biosynthetic production of [3 R ,4 R ]-4-amino-3-hydroxycyclohexa-1,5-diene-1-carboxylic acid (3,4-CHA), by concerted action of such 4-amino-4-deoxychorismate synthase and of an enzyme capable of converting isochorismate into [5 S ,6 S ]-5,6-dihydroxycyclohexa-1,3-diene-1-carboxylic acid (2,3-CHD), preferably a phenazine biosynthesis protein PhzD, including recovery of 3,4-CHA. The invention also relates to expression vectors and host cells for use in any of such processes. And the invention finally relates to synthesis of oseltamivir phosphate from 3,4-CHA.

IPC 8 full level

C12P 13/00 (2006.01); C12N 1/14 (2006.01); C12N 1/20 (2006.01); C12P 7/42 (2006.01); C12P 13/04 (2006.01)

CPC (source: EP KR US)

C12N 1/20 (2013.01 - KR); C12P 7/42 (2013.01 - KR); C12P 13/00 (2013.01 - KR); C12P 13/04 (2013.01 - EP KR US)

Citation (search report)

  • [YX] US 2003224495 A1 20031204 - FRANKE DIRK [DE], et al
  • [X] BLANC V ET AL: "IDENTIFICATION AND ANALYSIS OF GENES FROM STREPTOMYCES PRISTINAESPIRALIS ENCODING ENZYMES INVOLVED IN THE BIOSYNTHESIS OF THE 4-DIMETHYLAMINO-L-PHENYLALANINE PRECURSOR OF PRISTINAMYCIN I", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 23, no. 2, 1997, pages 191 - 202, XP002934995, ISSN: 0950-382X
  • [X] TENG, CHIA YU P. ET AL TENG, CHIA YU P. ET AL: "Total synthesis of (.+-.)-4-amino-4-deoxychorismic acid: a key intermediate in the biosynthesis of p-aminobenzoic acid and L-p-aminophenylalanine Total synthesis of (.+-.)-4-amino-4-deoxychorismic acid: a key intermediate in the biosynthesis of p-aminobenzoic acid and L-p-aminophenylalanine", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY , 107(17), 5008-9 CODEN: JACSAT; ISSN: 0002-7863 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY , 107(17), 5008-9 CODEN: JACSAT; ISSN: 0002-7863, 1985, XP002304925
  • [X] YE QI-ZHUANG ET AL.: "p-Aminobenzoate synthesis in Escherichia coli.", PROC. NATL. ACAD. SCI., vol. 87, 1990, pages 9391 - 9395, XP002304926
  • [X] NICHOLS B.P. ET AL.: "p-Aminobenzoate Synthesis from Chorismate Occurs in Two Steps.", J. BIOL. CHEM., vol. 264, no. 15, 1989, pages 8597 - 8601, XP002304927
  • [YD] PARSONS J F ET AL: "Structure and mechanism of Pseudomonas aeruginosa PhzD, and isochorismatase from the phenazine biosynthetic pathway", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 42, 2003, pages 5684 - 5693, XP002352900, ISSN: 0006-2960
  • [Y] BROWN M P ET AL: "A ROLE FOR PABAB, A P-AMINOBENZOATE SYNTHASE GENE OF STREPTOMYCES VENEZUELAE ISP5230, IN CHLORAMPHENICOL BIOSYNTHESIS", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, GB, vol. 142, no. PART 6, June 1996 (1996-06-01), pages 1345 - 1355, XP001203485, ISSN: 1350-0872

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR

DOCDB simple family (publication)

EP 1602730 A2 20051207; EP 1602730 A3 20060125; CN 101031652 A 20070905; EP 1751297 A2 20070214; JP 2008501326 A 20080124; KR 20070083447 A 20070824; US 2008311631 A1 20081218; WO 2005118829 A2 20051215; WO 2005118829 A3 20060615

DOCDB simple family (application)

EP 04076644 A 20040604; CN 200580026480 A 20050602; EP 05755137 A 20050602; EP 2005005988 W 20050602; JP 2007513866 A 20050602; KR 20077000170 A 20070103; US 62845905 A 20050602