EP 1613965 A2 20060111 - APPARATUS AND METHOD FOR DETECTING MICROSCOPIC LIVING ORGANISMS USING BACTERIOPHAGE
Title (en)
APPARATUS AND METHOD FOR DETECTING MICROSCOPIC LIVING ORGANISMS USING BACTERIOPHAGE
Title (de)
VORRICHTUNG UND VERFAHREN ZUM NACHWEIS MIKROSKOPISCHER LEBENDER ORGANISMEN MITTELS BAKTERIOPHAGEN
Title (fr)
APPAREIL ET PROCEDE DE DETECTION D'ORGANISMES VIVANTS MICROSCOPIQUES AU MOYEN DE BACTERIOPHAGE
Publication
Application
Priority
- US 2004011285 W 20040412
- US 24945203 A 20030410
- US 0311253 W 20030410
- US 54443704 P 20040213
- US 55796204 P 20040331
Abstract (en)
[origin: WO2005001475A2] A method for detecting one or more target bacteria (14) in a raw sample where: bacteriophage(s) (18, 102) specific to each target bacterium are added to the raw sample, the test sample is incubated, and the test sample is applied to a substrate (64, 220) which changes color if either the bacteriophage or a biological substance associated with the bacteriophage is present. The substrate contains antibodies (44, 228) that bind specifically to each phage. The bacteria in the test sample may be lysed by adding a microbial lysozyme (22) to the bacteriophage exposed sample. The parent phages (102) are tagged (105) in such a way that they can be separated from the progeny phage (106) prior to the detection process. The phage can by dissociated (94, 124) after the incubation process and the sample tested (99, 116) for the presence of individual capsid proteins (97, 72) or phage nucleic acids (74). The invention can be used (140) to test target bacteria for antibiotic resistance.
IPC 1-7
IPC 8 full level
G01N 33/558 (2006.01); G01N 33/569 (2006.01)
CPC (source: EP)
G01N 33/558 (2013.01); G01N 33/56911 (2013.01)
Citation (search report)
See references of WO 2005001475A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005001475 A2 20050106; WO 2005001475 A3 20050303; EP 1613965 A2 20060111
DOCDB simple family (application)
US 2004011285 W 20040412; EP 04775884 A 20040412