EP 1615999 A2 20060118 - NOVEL PROCESS FOR DETACHING CONFLUENT CELLS FROM TWO DIMENSIONAL MICROSUPPORTS AND ITS APPLICATION FOR PREPARING TRANSPLANTS
Title (en)
NOVEL PROCESS FOR DETACHING CONFLUENT CELLS FROM TWO DIMENSIONAL MICROSUPPORTS AND ITS APPLICATION FOR PREPARING TRANSPLANTS
Title (de)
NEUES VERFAHREN ZUR ABLÖSUNG KONFLUENTER ZELLEN VON ZWEIDIMENSIONALEN MIKROTRÄGERN UND DESSEN ANWENDUNG ZUR HERSTELLUNG VON TRANSPLANTATEN
Title (fr)
PROCEDE NOUVEAU POUR DETACHER DES CELLULES CONFLUENTES DE MICROSUPPORTS BIDIMENSIONNELS ET SON APPLICATION POUR PREPARER DES TRANSPLANTATIONS
Publication
Application
Priority
- IB 2004000930 W 20040305
- US 45260103 P 20030305
Abstract (en)
[origin: WO2004078956A2] The present invention relates to a process for detaching confluent anchoragedependent cells (ADCs') from two-dimensional microsupports (2D-MS) onto which these ADCs' are cultivated, particularly ADCs' which can be cultivated in a state suitable for transplantation, such as ADCs' derived from pancreatic cells. The invention further relates to a process for preparing reconstituted organoids from such detached confluent ADCs', which can be immortalized, according to the invention. The invention also relates to the use of organoids obtained by the process according to the invention for screening cell activity modulator or as therapeutic transplant for their administration in patients in need of such treatment. Finally, the present invention relates to a method for preventing or treating diabetes wherein pancreatic cells-derived organoids according to the invention are transplanted in a patient in need of such treatment.
IPC 1-7
IPC 8 full level
C12N 5/00 (2006.01); C12N 5/071 (2010.01)
CPC (source: EP)
C12N 5/0075 (2013.01); C12N 5/0676 (2013.01); C12N 2509/00 (2013.01); C12N 2510/04 (2013.01); C12N 2533/30 (2013.01); C12N 2539/10 (2013.01)
Citation (search report)
See references of WO 2004078956A2
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
WO 2004078956 A2 20040916; WO 2004078956 A3 20060921; EP 1615999 A2 20060118
DOCDB simple family (application)
IB 2004000930 W 20040305; EP 04717723 A 20040305