EP 1620568 B1 20081224 - ALLELE-SPECIFIC MUTATION DETECTION ASSAY
Title (en)
ALLELE-SPECIFIC MUTATION DETECTION ASSAY
Title (de)
VERFAHREN ZUR DETEKTION ALLELSPEZIFISCHER MUTATIONEN
Title (fr)
ESSAIS DE DETECTION DE MUTATIONS PROPRES AUX ALLELES
Publication
Application
Priority
- GB 2004001766 W 20040426
- GB 0309350 A 20030424
Abstract (en)
[origin: US7262032B2] The present invention relates to a method of detecting a base at a pre-determined position in a nucleic acid molecule by performing enzymatic detection reactions using base-specific detection oligomers, where each oligomer is specific for a particular base at the predetermined position, and then comparing the enzymatic detection reactions to determine which base is present at the position, with an enzyme-disabling agent being present during the enzymatic detection reaction. In preferred embodiments the enzymatic detection reaction is an oligomer elongation extension reaction, catalysed by, among others, polymerase or ligase. Also disclosed are methods of performing the assay in a liquid phase and on microarrays.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6827 (2013.01 - EP US); C12Q 1/6837 (2013.01 - EP US); C12Q 1/6869 (2013.01 - EP US)
C-Set (source: EP US)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2004094666 A1 20041104; AT E418621 T1 20090115; DE 602004018631 D1 20090205; DK 1620568 T3 20090330; EP 1620568 A1 20060201; EP 1620568 B1 20081224; US 2006088872 A1 20060427; US 7262032 B2 20070828
DOCDB simple family (application)
GB 2004001766 W 20040426; AT 04729459 T 20040426; DE 602004018631 T 20040426; DK 04729459 T 20040426; EP 04729459 A 20040426; US 25669405 A 20051021