EP 1620575 A4 20070207 - METHOD OF ELECTROCHEMICAL DETECTION OF SOMATIC CELL MUTATIONS
Title (en)
METHOD OF ELECTROCHEMICAL DETECTION OF SOMATIC CELL MUTATIONS
Title (de)
VERFAHREN ZUM ELEKTROCHEMISCHEN NACHWEIS SOMATISCHER ZELLMUTATIONEN
Title (fr)
PROCEDE DE DETECTION ELECTROCHIMIQUE DE MUTATIONS DE CELLULES SOMATIQUES
Publication
Application
Priority
- US 2004013222 W 20040430
- US 42929303 A 20030502
Abstract (en)
[origin: CA2524265A1] The present invention relates to the detection of somatic cell mutations, particularly as part of a method to screen for cancer or precancer. The disclosure includes techniques for extracting and isolating oligonucleotides from a patient and conducting hybridization assays. Preferred embodiments include a combination of the following steps: extracting a biological sample from a patient, purifying a nucleic acid from a biological sample, amplifyin g a nucleic acid, isolating a nucleic acid in single stranded form, cyclizing a nucleic acid, elongating a nucleic acid, controlling hybridization stringenc y, amplifying a nucleic acid on a chip, and detecting hybridization.
IPC 8 full level
C12Q 1/68 (2006.01); C07H 21/04 (2006.01); C12P 19/34 (2006.01)
CPC (source: EP)
C12Q 1/6825 (2013.01); C12Q 1/6827 (2013.01)
Citation (search report)
- [X] US 2003073122 A1 20030417 - SOSNOWSKI RONALD G [US], et al
- [XY] WO 02083839 A2 20021024 - MOTOROLA INC [US]
- [Y] US 2002168645 A1 20021114 - TAYLOR SETH [US]
- [A] STEEL A B ET AL: "Electrochemical Quantitation of DNA immobilized on Gold", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 70, no. 22, 15 November 1998 (1998-11-15), pages 4670 - 4677, XP002285670, ISSN: 0003-2700
- See references of WO 2004099755A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
CA 2524265 A1 20041118; EP 1620575 A2 20060201; EP 1620575 A4 20070207
DOCDB simple family (application)
CA 2524265 A 20040430; EP 04760623 A 20040430