EP 1624751 A2 20060215 - CRYOPRESERVATION OF HUMAN BLASTOCYST-DERIVED STEM CELLS BY USE OF A CLOSED STRAW VITRIFICATION METHOD
Title (en)
CRYOPRESERVATION OF HUMAN BLASTOCYST-DERIVED STEM CELLS BY USE OF A CLOSED STRAW VITRIFICATION METHOD
Title (de)
KRYOKONSERVIERUNG HUMANER VON BLASTOZYSTEN ABGELEITETER STAMMZELLEN MITTELS EINES VERGLASUNGSVERFAHRENS, DAS GESCHLOSSENE RÖHRCHEN VERWENDET
Title (fr)
CRYOCONSERVATION DE CELLULES SOUCHES HUMAINES DERIVEES DE BLASTOCYSTES AU MOYEN D'UN PROCEDE DE VITRIFICATION DANS DES PAILLETTES FERMEES
Publication
Application
Priority
- EP 2004005031 W 20040510
- DK PA200300700 A 20030508
- US 46932003 P 20030508
- DK PA200300984 A 20030627
- DK PA200300983 A 20030627
Abstract (en)
[origin: WO2004098285A2] An improved method for vitrification of biological cells, especially blastocyst-derived stem cells (BS cells). The method is very mild for the cells that remain viable after they have been thawed. The method comprises, i) transfer of the cells to a first solution (solution A), ii) optionally incubation of the cells in the first solution, iii) transfer the cells obtained in step i) or ii) to a second solution (solution B), iv) optionally incubation of the cells in the second solution, v) transfer of the cells obtained from step iii) or iv) into one or more closed straws with dimensions that allow a volume of at least 20 µl to be contained in them vi) sealing the one or more closed straws, and vii) vitrification of the one or more closed straws. An important feature of the present invention is the use of closed straw and that relatively large volumes can be efficiently vitrified and subsequently thawed.
IPC 1-7
IPC 8 full level
A01N 1/02 (2006.01); C12N 5/0797 (2010.01); A61K 35/12 (2015.01)
CPC (source: EP GB KR)
A01N 1/02 (2013.01 - EP GB KR); A01N 1/0221 (2013.01 - EP); A61D 19/04 (2013.01 - GB); C12N 5/0623 (2013.01 - EP GB); A61K 35/12 (2013.01 - EP); C12N 2501/11 (2013.01 - EP); C12N 2501/115 (2013.01 - EP); C12N 2506/02 (2013.01 - EP); C12N 2533/52 (2013.01 - EP); C12N 2533/54 (2013.01 - EP)
Citation (search report)
See references of WO 2004098285A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2004098285 A2 20041118; WO 2004098285 A3 20050106; AU 2004237425 A1 20041118; AU 2004237425 B2 20100304; CA 2524929 A1 20041118; CA 2524929 C 20150113; EP 1624751 A2 20060215; GB 0525046 D0 20060118; GB 2417961 A 20060315; GB 2417961 A8 20080509; GB 2417961 B 20080312; GB 2417961 B8 20080509; IL 171595 A 20120731; JP 2006525006 A 20061109; KR 20060032953 A 20060418; SE 0502397 L 20051229
DOCDB simple family (application)
EP 2004005031 W 20040510; AU 2004237425 A 20040510; CA 2524929 A 20040510; EP 04731935 A 20040510; GB 0525046 A 20040510; IL 17159505 A 20051027; JP 2006505414 A 20040510; KR 20057020730 A 20051101; SE 0502397 A 20051028