Global Patent Index - EP 1631577 A4

EP 1631577 A4 20080723 - METHOD AND APPARATUS FOR RECOGNIZING MOLECULAR COMPOUNDS

Title (en)

METHOD AND APPARATUS FOR RECOGNIZING MOLECULAR COMPOUNDS

Title (de)

VERFAHREN UND GERÄT ZUR ERKENNUNG MOLEKULARER VERBINDUNGEN

Title (fr)

PROCEDE ET DISPOSITIF POUR RECONNAITRE DES COMPOSES MOLECULAIRES

Publication

EP 1631577 A4 20080723 (EN)

Application

EP 03741837 A 20030528

Priority

  • US 0317137 W 20030528
  • US 15899503 A 20030528

Abstract (en)

[origin: WO2004106357A1] A probe-target reaction is made more recognizable by the provision of a mass-enhancing and/or evanescent-field-perturbing amplifier element which reacts uniquely with and binds to the probe-target pair to provide increased mass. Where the probe-target pair is hybridized dsDNA, a suitable mass­ enhancing amplifier is anti-double stranded DNA mouse IgM. In examples with sufficient sequence pairs in the probe-target combination, a sequence-specific minor-groove-binding polyamide can be used that carries biotin which can be amplified by streptavidin in a suitable carrier. In a preferred embodiment, a plurality of probes are immobilized at the sites of a microarray, each probe being specific to a different target. Optics utilizing total internal reflection are described for observing perturbation of the evanescent field.

IPC 8 full level

C07H 21/04 (2006.01); C12M 3/00 (2006.01); C12Q 1/68 (2006.01); G01J 4/00 (2006.01); G01N 21/00 (2006.01); G01N 21/17 (2006.01); G01N 21/21 (2006.01); G01N 21/55 (2006.01); G01N 21/75 (2006.01); G01N 33/00 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/557 (2006.01)

CPC (source: EP)

C07H 21/04 (2013.01); G01N 21/21 (2013.01); G01N 21/552 (2013.01); G01N 33/54373 (2013.01); G01N 2021/757 (2013.01)

Citation (search report)

  • [XY] US 4608344 A 19860826 - CARTER TIMOTHY J N [GB], et al
  • [X] WO 9809156 A1 19980305 - CIBA GEIGY AG [CH], et al
  • [T] WO 2005035790 A1 20050421 - MAVEN TECHNOLOGIES LLC [US]
  • [Y] WO 9837066 A1 19980827 - CALIFORNIA INST OF TECHN [US], et al
  • [Y] BOHRMANN B ET AL: "Immunostraining of DNA in electron microscopy: an amplification and straining procedure for thin sections aa alternative to gold labeling", JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY, HISTOCHEMICAL SOCIETY, NEW YORK, NY, US, vol. 42, no. 5, 1994, pages 635 - 643, XP008030957, ISSN: 0022-1554
  • [X] SCHNEIDER B H ET AL: "Optical chip immunoassay for hCG in human whole blood", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 15, no. 11-12, December 2000 (2000-12-01), pages 597 - 604, XP002281252, ISSN: 0956-5663
  • [X] SCHNEIDER B H ET AL: "Hartman interferometer: Versatile integrated optic sensor for label-free, real-time quantification of nucleic acids, proteins, and pathogens", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY, WASHINGTON, DC, US, vol. 43, no. 9, 1997, pages 1757 - 1763, XP002281012, ISSN: 0009-9147
  • [X] POLZIUS R ET AL: "Real-time observation of affinity reactions using grating couplers: Determination of the detection limit and calculation of kinetic rate constants", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 248, no. 2, 1997, pages 269 - 276, XP002281014, ISSN: 0003-2697
  • [X] BIER F F ET AL: "Label-free observation of DNA-hybridisation and endonuclease activity on a wave guide surface using a grating coupler", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 11, no. 6-7, 1996, pages 669 - 674, XP002281013, ISSN: 0956-5663
  • [XY] WINK T ET AL: "Liposome-mediated enhancement of the sensitivity in immunoassays of proteins and peptides in surface plasmon resonance spectrometry", 1 March 1998, ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, PAGE(S) 827-832, ISSN: 0003-2700, XP002206192
  • [XY] HE L ET AL: "Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 122, 2000, pages 9071 - 9077, XP002206375, ISSN: 0002-7863
  • See references of WO 2004106357A1

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

DOCDB simple family (publication)

WO 2004106357 A1 20041209; AU 2003304150 A1 20050121; CN 1823085 A 20060823; EP 1631577 A1 20060308; EP 1631577 A4 20080723; JP 2007528692 A 20071018

DOCDB simple family (application)

US 0317137 W 20030528; AU 2003304150 A 20030528; CN 03826863 A 20030528; EP 03741837 A 20030528; JP 2005500431 A 20030528