Global Patent Index - EP 1656456 A1

EP 1656456 A1 20060517 - METHOD OF SEPARATING OPTICAL ISOMERS OF A PROTECTED AMINOACID

Title (en)

METHOD OF SEPARATING OPTICAL ISOMERS OF A PROTECTED AMINOACID

Title (de)

VERFAHREN ZUR TRENNUNG OPTISCHER ISOMERE EINER GESCHÜTZTEN AMINOSÄURE

Title (fr)

PROCEDE DE SEPARATION D'ISOMERES OPTIQUES D'UN ACIDE AMINE PROTEGE

Publication

EP 1656456 A1 20060517 (EN)

Application

EP 04748942 A 20040625

Priority

  • RU 2004000244 W 20040625
  • NL 1023767 A 20030627

Abstract (en)

[origin: WO2005001107A1] The invention relates to a method Method of enzy­matically separating optical isomers of a protected amino­ acid. According to the invention, a (non-sidechain-carboxyl)­ protected aminoacid is used having the general formula (I) HR<1>N-CR<2>- (CH) n-C (0) ZR<3 >wherein R<1> is an amino-protective group or hydrogen; - R<2> is a side chain; n is a number chosen from 0, 1, and 2; ZR<3> is a carboxy-protective group where Z is chosen from 0 or NH; and R<1> and R<3> may be integrated, forming a heterocy­clic (non-sidechain-carboxyl)-protected ring, the ring backbone comprising 5 to 8 atoms; the (non-sidechain-carboxyl)-protected aminoacid having a mo­lecular weight of less than 1000 Dalton, said method comprising the steps of - contacting the (non-sidechain-carboxyl) -protected aminoacid (I) in the presence of water with an optionally partially pu­rified cell-free extract of a fungus containing an enzyme having at least one of an esterase and amidase activity yielding a mixture of (non-sidechain carboxyl) deprotected product and starting material; and - optionally separating the (non-sidechain carboxyl) depro­tected product and starting material.

IPC 1-7

C12P 41/00; C07C 227/34

IPC 8 full level

C07K 14/38 (2006.01); C12P 13/04 (2006.01); C12P 41/00 (2006.01)

CPC (source: EP)

C07K 14/38 (2013.01); C12P 13/04 (2013.01); C12P 41/005 (2013.01); C12P 41/007 (2013.01); Y02P 20/55 (2015.11)

Citation (search report)

See references of WO 2005001107A1

Designated contracting state (EPC)

DE FR NL

DOCDB simple family (publication)

WO 2005001107 A1 20050106; EP 1656456 A1 20060517; NL 1023767 C1 20041228; RU 2006100556 A 20070827

DOCDB simple family (application)

RU 2004000244 W 20040625; EP 04748942 A 20040625; NL 1023767 A 20030627; RU 2006100556 A 20040625