EP 1656456 A1 20060517 - METHOD OF SEPARATING OPTICAL ISOMERS OF A PROTECTED AMINOACID
Title (en)
METHOD OF SEPARATING OPTICAL ISOMERS OF A PROTECTED AMINOACID
Title (de)
VERFAHREN ZUR TRENNUNG OPTISCHER ISOMERE EINER GESCHÜTZTEN AMINOSÄURE
Title (fr)
PROCEDE DE SEPARATION D'ISOMERES OPTIQUES D'UN ACIDE AMINE PROTEGE
Publication
Application
Priority
- RU 2004000244 W 20040625
- NL 1023767 A 20030627
Abstract (en)
[origin: WO2005001107A1] The invention relates to a method Method of enzymatically separating optical isomers of a protected amino acid. According to the invention, a (non-sidechain-carboxyl) protected aminoacid is used having the general formula (I) HR<1>N-CR<2>- (CH) n-C (0) ZR<3 >wherein R<1> is an amino-protective group or hydrogen; - R<2> is a side chain; n is a number chosen from 0, 1, and 2; ZR<3> is a carboxy-protective group where Z is chosen from 0 or NH; and R<1> and R<3> may be integrated, forming a heterocyclic (non-sidechain-carboxyl)-protected ring, the ring backbone comprising 5 to 8 atoms; the (non-sidechain-carboxyl)-protected aminoacid having a molecular weight of less than 1000 Dalton, said method comprising the steps of - contacting the (non-sidechain-carboxyl) -protected aminoacid (I) in the presence of water with an optionally partially purified cell-free extract of a fungus containing an enzyme having at least one of an esterase and amidase activity yielding a mixture of (non-sidechain carboxyl) deprotected product and starting material; and - optionally separating the (non-sidechain carboxyl) deprotected product and starting material.
IPC 1-7
IPC 8 full level
C07K 14/38 (2006.01); C12P 13/04 (2006.01); C12P 41/00 (2006.01)
CPC (source: EP)
C07K 14/38 (2013.01); C12P 13/04 (2013.01); C12P 41/005 (2013.01); C12P 41/007 (2013.01); Y02P 20/55 (2015.11)
Citation (search report)
See references of WO 2005001107A1
Designated contracting state (EPC)
DE FR NL
DOCDB simple family (publication)
WO 2005001107 A1 20050106; EP 1656456 A1 20060517; NL 1023767 C1 20041228; RU 2006100556 A 20070827
DOCDB simple family (application)
RU 2004000244 W 20040625; EP 04748942 A 20040625; NL 1023767 A 20030627; RU 2006100556 A 20040625