EP 1658496 A1 20060524 - PHOTOCHROMIC RELAXATION KINETIC METHOD
Title (en)
PHOTOCHROMIC RELAXATION KINETIC METHOD
Title (de)
PHOTOCHROMES RELAXATIONSKINETISCHES VERFAHREN
Title (fr)
PROCEDE A CINETIQUE DE RELAXATION PHOTOCHROMIQUE
Publication
Application
Priority
- EP 2004008729 W 20040804
- DE 10337108 A 20030811
Abstract (en)
[origin: WO2005017527A1] The invention relates to a method for determining a characteristic kinetic variable of a chemical reaction between a plurality of chemical species in a sample, at least one species comprising at least one fluorophore. According to said method, an imbalance state of the chemical reaction is generated by exposing the sample to light and by temporally resolved observation of at least one section of the relaxation of the concentrations of the species involved, by means of a fluorescence signal of at least one fluorophore. According to the invention, at least one product of the chemical reaction to be examined comprises a compound of two species, that respectively contain a partner of a FRET pair consisting of a FRET donor and a FRET acceptor. The invention is characterised by further steps: the FRET acceptor is a photochrome having an absorption spectrum that can be modified by means of exposition to light of a suitable wavelength; the FRET donor is a fluorophore having an emission spectrum which overlaps with the absorption spectrum of the FRET acceptor, the size of said region depending on the photochromic state of the FRET acceptor; and the light used for generating the imbalance state of the chemical reaction has a wavelength which changes the photochromic state of the FRET acceptor.
IPC 1-7
IPC 8 full level
G01N 33/542 (2006.01)
CPC (source: EP US)
G01N 33/542 (2013.01 - EP US)
Citation (search report)
See references of WO 2005017527A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005017527 A1 20050224; DE 10337108 B3 20050504; EP 1658496 A1 20060524; IL 173643 A0 20060705; JP 2007501936 A 20070201; US 2007184445 A1 20070809
DOCDB simple family (application)
EP 2004008729 W 20040804; DE 10337108 A 20030811; EP 04763779 A 20040804; IL 17364306 A 20060209; JP 2006522955 A 20040804; US 56803804 A 20040804