EP 1666150 A1 20060607 - Nucleic acid preparation
Title (en)
Nucleic acid preparation
Title (de)
Präparieren von Nucleinsäuren
Title (fr)
Préparation d'acide nucléique
Publication
Application
Priority
- EP 04027624 A 20041120
- EP 05024993 A 20051116
Abstract (en)
The present invention is directed to methods, devices and computer programs for preparing nucleic acids from a template nucleic acid by subjecting a sample to thermocycles. After a first number of thermocycles, a partial amount of the reaction mixture is being subjected to a second number of thermocycles. This two step amplification method allows to speed up overall reaction time without affecting the limit of detection.
IPC 8 full level
CPC (source: EP)
B01L 7/52 (2013.01); B01L 3/5021 (2013.01); B01L 2300/0803 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1861 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0688 (2013.01)
Citation (applicant)
- EP 0201184 A2 19861112 - CETUS CORP [US]
- EP 0200362 A2 19861105 - CETUS CORP [US]
- EP 0543942 A1 19930602 - HOFFMANN LA ROCHE [CH]
- WO 2004051218 A2 20040617 - APPLERA CORP [US], et al
- WO 0220845 A2 20020314 - UNIV JEFFERSON [US], et al
- US 4683202 A 19870728 - MULLIS KARY B [US]
- US 5185243 A 19930209 - ULLMAN EDWIN F [US], et al
- US 5679524 A 19971021 - NIKIFOROV THEO [US], et al
- US 5573907 A 19961112 - CARRINO JOHN J [US], et al
- WO 03106031 A1 20031224 - HOFFMANN LA ROCHE [CH], et al
- EP 0543942 A1 19930602 - HOFFMANN LA ROCHE [CH]
- US 2003152492 A1 20030814 - CHANG RONALD [US], et al
- US 5639423 A 19970617 - NORTHRUP M ALLEN [US], et al
- SAMBROOK: "MOLECULAR CLONING - A LABORATORY MANUAL", 1989, COLDSPRING HARBOUR LABORATORY PRESS
- LEE, J. MICROMECH. MICROENG., vol. 13, 2003, pages 89 - 97
- HANDIQUE, ANAL. CHEM., vol. 72, pages 4100 - 9
- HOSOKAWA, ANAL. CHEM., vol. 71, no. 1, pages 4781 - 5
- PUNTAMBEKAR: "PROC. TRANSDUCERS'01", BERLIN: SPRINGER, pages: 1240 - 3
- ZHAO, SCIENCE, vol. 291, pages 1023 - 6
- ANDERSSON, SENSORS ACTUATORS, vol. 75, pages 136 - 41
- A VAN DEN BERG, P BERVELD: "Micro Total Analysis Systems", PROCEEDINGS UTAS'94, 1994
- M J HELLER; A GUTTMAN, INTEGRATED MICROFABRICATED BIODEVICES, 2002
- O GESCHKE; H KLANK; P TELLEMANN, MICROSYSTEM ENGINEERING OF LAB-ON-A-CHIP DEVICES, 2004
Citation (search report)
- [A] US 2002127708 A1 20020912 - KLUTTZ BRYAN W [US], et al
- [AD] WO 0220845 A2 20020314 - UNIV JEFFERSON [US], et al
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA HR MK YU
DOCDB simple family (publication)
DOCDB simple family (application)
EP 05024993 A 20051116