EP 1666150 B1 20150107 - Nucleic acid preparation
Title (en)
Nucleic acid preparation
Title (de)
Präparieren von Nucleinsäuren
Title (fr)
Préparation d'acide nucléique
Publication
Application
Priority
- EP 04027624 A 20041120
- EP 05024993 A 20051116
Abstract (en)
[origin: EP1666150A1] The present invention is directed to methods, devices and computer programs for preparing nucleic acids from a template nucleic acid by subjecting a sample to thermocycles. After a first number of thermocycles, a partial amount of the reaction mixture is being subjected to a second number of thermocycles. This two step amplification method allows to speed up overall reaction time without affecting the limit of detection.
IPC 8 full level
CPC (source: EP)
B01L 7/52 (2013.01); B01L 3/5021 (2013.01); B01L 2300/0803 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1861 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0688 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
DOCDB simple family (application)
EP 05024993 A 20051116