EP 1670929 A2 20060621 - METHOD FOR PRODUCING A CAROTINOID-CONTAINING BIOMASS
Title (en)
METHOD FOR PRODUCING A CAROTINOID-CONTAINING BIOMASS
Title (de)
VERFAHREN ZUR HERSTELLUNG CAROTINOIDHALTIGER BIOMASSE
Title (fr)
PROCEDE DE PRODUCTION DE BIOMASSE A BASE DE CAROTINOIDE
Publication
Application
Priority
- DE 2004002162 W 20040926
- DE 10345372 A 20030926
Abstract (en)
[origin: WO2005030976A2] The invention relates to the production of a carotinoid-containing biomass. The aim of the invention is to provide a method for producing carotinoid-containing biomass that can be realized with a low level of complexity on a large scale and without antibiotics. To this end, the invention provides that non-genetically modified heterothallic fungi with an increased synthesis and storage capacity of ss-carotene are cultured in submerged cultures, whereby the biomass is propagated separately according to (+) and (-) pair type in multiple stages exclusively from spores. Biomass of the (+) pair type or culture liquid of the (+) pair type or homogenized biomass of the (+) pair type or, instead of the (+) pair type, gamone-type stimulators or lactones or trisporic acid or trisporic acid derivatives is/are added to the biomass of the (-) pair type after completion of the last biomass propagation phase before the production fermentation, and this submerged mixture is transferred into the nutrient solution of the product fermentation after a maturing time lasting form hours. The nutrient solution is composed in such a manner that at time tw, the growth phase of the biomass is terminated by phosphorous limitation, the synthesis of ss-carotene is additionally triggered by creating a short-term stress situation, and the solvent-free extraction of the ss-carotene from the resulting dry biomass is made possible by a phosphorous limitation during the growth phase.
IPC 1-7
IPC 8 full level
C12P 23/00 (2006.01)
CPC (source: EP)
C12P 23/00 (2013.01)
Citation (search report)
See references of WO 2005030976A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005030976 A2 20050407; WO 2005030976 A3 20050929; CN 1886519 A 20061227; DE 112004002328 D2 20060810; EP 1670929 A2 20060621
DOCDB simple family (application)
DE 2004002162 W 20040926; CN 200480034773 A 20040926; DE 112004002328 T 20040926; EP 04786874 A 20040926