EP 1673381 A2 20060628 - SOLID PHASE METHODS FOR POLYNUCLEOTIDE PRODUCTION
Title (en)
SOLID PHASE METHODS FOR POLYNUCLEOTIDE PRODUCTION
Title (de)
FESTPHASENVERFAHREN ZUR POLYNUKLEOTIDPRODUKTION
Title (fr)
PROCEDE DE PRODUCTION, EN PHASE SOLIDE, DE POLYNUCLEOTIDES
Publication
Application
Priority
- US 2004033919 W 20041013
- US 51177003 P 20031015
- US 51214103 P 20031016
Abstract (en)
[origin: WO2005037850A2] Polynucleotides having in excess of 1,000 nucleotides can be prepared using a solid phase synthesis technique. A feature of the technique is the use of a reusable solid support that contains covalently bound oligonucleotide. This covalently bound oligonucleotide is annealed to a bridge oligonucleotide, where the bridge is also annealed to a first oligonucleotide that forms a portion of the target polynucleotide. After the target polynucleotide is synthesized, it can be removed from the solid support under denaturing conditions, and the solid support re-used to prepare additional target polynucleotides. The yield of the target polynucleotide increases when shearing force is applied to the solid support that is linked to the growing oligonucleotide. This shearing force is thought to extend the growing end of the oligonucleotide away from contact with other oligonucleotide bound to the solid support and make that end more accessible to annealing with solution oligonucleotide. The synthesis is conveniently accomplished on a porous frit, where reagents and washing solutions are pumped through the frit.
IPC 1-7
IPC 8 full level
C07H 21/00 (2006.01); C12P 19/34 (2006.01)
CPC (source: EP)
C07H 21/00 (2013.01); C12P 19/34 (2013.01)
Citation (search report)
See references of WO 2005037850A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005037850 A2 20050428; WO 2005037850 A3 20050909; CA 2543014 A1 20050428; EP 1673381 A2 20060628; JP 2007508825 A 20070412
DOCDB simple family (application)
US 2004033919 W 20041013; CA 2543014 A 20041013; EP 04809957 A 20041013; JP 2006535658 A 20041013