EP 1675943 A4 20071205 - NANOLITER-SCALE SYNTHESIS OF ARRAYED BIOMATERIALS AND SCREENING THEREOF
Title (en)
NANOLITER-SCALE SYNTHESIS OF ARRAYED BIOMATERIALS AND SCREENING THEREOF
Title (de)
SYNTHESE VON IN EINEM ARRAY ANGEORDNETEN BIOMATERIALIEN IM NANOLITERMASSSTAB UND SCREENING DAVON
Title (fr)
SYNTHESE, A L'ECHELLE DU NANOLITRE, DE BIOMATERIAUX EN RESEAUX ET CRIBLAGE DE CEUX-CI
Publication
Application
Priority
- US 2004030095 W 20040915
- US 50316503 P 20030915
- US 57018704 P 20040512
- US 84370704 A 20040512
Abstract (en)
[origin: WO2005028619A2] A population of embryonic epithelial cells produced in vitro from embryonic stem cells. In one embodiment, at least 45% of the cells express cytokeratin, for example, cytokeratin-7. A method of screening cell-polymer interactions. The method includes depositing monomers as a plurality of discrete elements on a substrate, causing the deposited monomers to polymerize, thereby creating an array of discrete polymer elements on the substrate, incubating the substrate in a cell-containing culture medium, and characterizing a predetermined cell behavior on each polymer element.
IPC 8 full level
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/071 (2010.01); G01N 33/50 (2006.01)
IPC 8 main group level
C12N (2006.01)
CPC (source: EP)
C12N 5/0606 (2013.01); G01N 33/5073 (2013.01); C12N 2503/02 (2013.01); C12N 2533/30 (2013.01); G01N 2500/10 (2013.01)
Citation (search report)
- [X] WO 02097068 A2 20021205 - INST NAT SANTE RECH MED [FR], et al
- [X] ALI N N ET AL: "DERIVATION OF TYPE II ALVEOLAR EPITHELIAL CELLS FROM MURINE EMBRYONIC STEM CELLS", TISSUE ENGINEERING, LARCHMONT, NY, US, vol. 8, no. 4, August 2002 (2002-08-01), pages 541 - 550, XP008025656, ISSN: 1076-3279
- [Y] CHEN RUTH R ET AL: "Polymeric growth factor delivery strategies for tissue engineering.", PHARMACEUTICAL RESEARCH AUG 2003, vol. 20, no. 8, August 2003 (2003-08-01), pages 1103 - 1112, XP002454034, ISSN: 0724-8741
- [Y] SAKIYAMA-ELBERT SE ET AL.: "Functional Biomaterials:design of novel biomaterials", ANNU.REV.MATER.RES., vol. 31, 2001, pages 183 - 201, XP009090540
- [A] SPRADLING ALLAN ET AL: "STEM CELLS FIND THEIR NICHE", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 414, no. 6859, November 2001 (2001-11-01), pages 98 - 104, XP009078342, ISSN: 0028-0836
- [A] LYLE S ET AL: "Cytokeratin 15(K15) as an epithelial stem cell marker: Implication for aging and carcinogenesis", JOURNAL OF INVESTIGATIVE DERMATOLOGY, NEW YORK, NY, US, vol. 112, no. 4, 5 May 1999 (1999-05-05), pages 623, XP003010867, ISSN: 0022-202X
- [PX] ANDERSON DANIEL G ET AL: "Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells.", NATURE BIOTECHNOLOGY JUL 2004, vol. 22, no. 7, July 2004 (2004-07-01), pages 863 - 866, XP002454174, ISSN: 1087-0156
- See references of WO 2005028619A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005028619 A2 20050331; WO 2005028619 A3 20050909; CA 2539670 A1 20050331; EP 1675943 A2 20060705; EP 1675943 A4 20071205
DOCDB simple family (application)
US 2004030095 W 20040915; CA 2539670 A 20040915; EP 04788758 A 20040915