EP 1692155 A2 20060823 - SYSTEM AND METHODS FOR NUCLEIC ACID AND POLYPEPTIDE SELECTION
Title (en)
SYSTEM AND METHODS FOR NUCLEIC ACID AND POLYPEPTIDE SELECTION
Title (de)
SYSTEM UND VERFAHREN ZUR AUSWAHL VON NUKLEINSÄUREN UND POLYPEPTIDEN
Title (fr)
SYSTEMES ET PROCEDES POUR LA SELECTION D'ACIDE NUCLEIQUE ET DE POLYPEPTIDE
Publication
Application
Priority
- US 2004041380 W 20041210
- US 52933103 P 20031212
- US 84708704 A 20040517
- US 84748404 A 20040517
- US 62570704 P 20041105
Abstract (en)
[origin: WO2005072087A2] This invention relates generally to systems and methods for identifying and selecting, desired proteins or nucleic acid molecules by linking mRNA, with known or unknown sequences, to its translated protein to form a cognate pair. The cognate pair is selected based upon desired properties of the protein or the nucleic acid. This method also includes the evolution of a desired protein or nucleic acid molecule by amplifying the nucleic acid portion of the selected cognate pair, introducing variation into the nucleic acid, translating the nucleic acid, attaching the nucleic acid to its protein to form a second cognate pair, and re-selecting this cognate pair based upon desired properties. Modified mRNAs operable to crosslink to tRNAs are also provided. Methods of producing a psoralen monoadduct or a crosslink are also provided. Methods of producing mRNA libraries and vaccines are also provided.
IPC 8 full level
C12N 15/10 (2006.01); C07H 21/04 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
A61P 11/00 (2017.12); A61P 35/00 (2017.12); C12N 15/1062 (2013.01); C12Q 1/6811 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005072087 A2 20050811; WO 2005072087 A3 20051103; AU 2004314705 A1 20050811; CA 2548465 A1 20050811; EP 1692155 A2 20060823; EP 1692155 A4 20080806; JP 2007513631 A 20070531
DOCDB simple family (application)
US 2004041380 W 20041210; AU 2004314705 A 20041210; CA 2548465 A 20041210; EP 04821258 A 20041210; JP 2006544008 A 20041210