EP 1706505 A2 20061004 - METHOD FOR VALIDATING AND/OR CALIBRATING A SYSTEM FOR PERFORMING HYBRIDISATION EXPERIMENTS, MICROARRAY, AND KIT THEREFOR
Title (en)
METHOD FOR VALIDATING AND/OR CALIBRATING A SYSTEM FOR PERFORMING HYBRIDISATION EXPERIMENTS, MICROARRAY, AND KIT THEREFOR
Title (de)
VERFAHREN ZUM VALIDIEREN UND/ODER KALIBRIEREN EINES SYSTEMS ZUR DURCHFÜHRUNG VON HYBRIDISIERUNGSEXPERIMENTEN, MIKROARRAY UND KIT HIERFÜR
Title (fr)
PROCEDE POUR VALIDER ET/OU CALIBRER UN SYSTEME POUR L'EXECUTION D'EXPERIENCES D'HYBRIDATION, JEU ORDONNE DE MICRO-ECHANTILLONS ET NECESSAIRE CORRESPONDANTS
Publication
Application
Priority
- EP 2004014414 W 20041217
- DE 10361137 A 20031223
Abstract (en)
[origin: WO2005064012A2] The invention relates to a method for calibrating and/or validating a system for performing a microarray experiment, in addition to a microarray, and a kit therefor. The inventive method comprises the following steps: a) a microarray is prepared, said microarray comprising at least one first measuring point containing probe molecules (PM) that are complementary to the target molecules and can form, with the target molecules, hybrids that have a melting temperature Tm(PM), and at least one second measuring point containing probe molecules (MM1) that are partially complementary to the target molecules and can form, with the target molecules, partial hybrids that have a melting temperature Tm(MM1); b) target molecules are provided, that are complementary to probe molecules (PM) in at least one first measuring point of the microarray; c) a hybridisation experiment is performed with the microarray and the target molecules, with variation of a defined parameter; and d) the signal intensities are determined according to the variation of the parameter. The parameter value at the maximum difference between the signal intensities of the first and second measuring points is the parameter value for which the system is approximately in equilibrium in relation to the hybrids in the first measuring point, and according to which the system is calibrated.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/6837 (2013.01); B01J 2219/00693 (2013.01)
Citation (search report)
See references of WO 2005064012A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005064012 A2 20050714; WO 2005064012 A3 20050909; DE 10361137 A1 20050728; EP 1706505 A2 20061004
DOCDB simple family (application)
EP 2004014414 W 20041217; DE 10361137 A 20031223; EP 04804017 A 20041217