EP 1707260 A1 20061004 - Method of preparing crosslinked collagen microspheres
Title (en)
Method of preparing crosslinked collagen microspheres
Title (de)
Verfahren zur Herstellung von vernetzten Mikrosphären aus Collagen
Title (fr)
Procédé pour la préparation de microsphère de collagen reticulé
Publication
Application
Priority
US 9580205 A 20050331
Abstract (en)
The present invention is drawn to methods of preparing crosslinked collagen microspheres and microparticles, where a solution of soluble collagen in an aqueous acidic medium is combined with an emulsifying agent to form a first mixture of the soluble collagen solution and the emulsifying agent, the first mixture then is combined with a hydrophobic liquid under conditions effective to form water-in-oil emulsion of the hydrophobic liquid and the first mixture, the water-in-oil emulsion then is added to a buffered alcohol solution under conditions effective to form a dispersion of a continuous liquid phase and a dispersed solid phase of collagen fibers reconstituted from the soluble collagen solution, the liquid phase then is removed from the dispersion to provide collagen microspheres, the collagen microspheres then are crosslinked to form crosslinked collagen microspheres, and the crosslinked collagen microspheres then are optionally dried to form crosslinked collagen microparticles.
IPC 8 full level
B01J 13/14 (2006.01)
CPC (source: EP US)
B01J 13/14 (2013.01 - EP US)
Citation (search report)
- [A] EP 0461631 A2 19911218 - TOSHIBA KK [JP], et al
- [DA] US 5672301 A 19970930 - ORLY ISABELLE [FR], et al
- [A] HSU F Y ET AL: "Microspheres of hydroxyapatite/reconstituted collagen as supports for osteoblast cell growth.", BIOMATERIALS. OCT 1999, vol. 20, no. 20, October 1999 (1999-10-01), pages 1931 - 1936, XP002388329, ISSN: 0142-9612
Designated contracting state (EPC)
DE ES FR GB IT NL
DOCDB simple family (publication)
EP 1707260 A1 20061004; AU 2006201334 A1 20061019; CA 2541269 A1 20060930; JP 2006291198 A 20061026; US 2006222680 A1 20061005
DOCDB simple family (application)
EP 06251758 A 20060330; AU 2006201334 A 20060330; CA 2541269 A 20060330; JP 2006094574 A 20060330; US 9580205 A 20050331