EP 1713910 A1 20061025 - METHOD FOR CHROMATOGRAPHIC SEPARATION OF A NUCLEIC ACID MIXTURE
Title (en)
METHOD FOR CHROMATOGRAPHIC SEPARATION OF A NUCLEIC ACID MIXTURE
Title (de)
VERFAHREN ZUR CHROMATOGRAPHISCHEN TRENNUNG EINES NUKLEINSÄUREGEMISCHES
Title (fr)
PROCEDE DE SEPARATION CHROMATOGRAPHIQUE D'UN MELANGE D'ACIDES NUCLEIQUES
Publication
Application
Priority
- EP 2005000693 W 20050125
- EP 04001864 A 20040129
- EP 05706998 A 20050125
Abstract (en)
[origin: EP1559783A1] Method for chromatographic separation of a nucleic acid (NA) mixture so that plasmid-DNA (I) is separated from other components, especially other NA, is new. Method for chromatographic separation of a nucleic acid (NA) mixture so that plasmid-DNA (I) is separated from other components, especially other NA, comprises: (A) optionally adjusting the conductivity of the starting mixture to 70-95 mS at pH 4.8-5.4 and 20[deg]C by adding one or more alkali and/or alkaline earth salts in an aqueous solution; (B) applying the mixture to a chromatographic material; (C) washing at least once with a solution of 0.9-1.8 M alkali salt and/or 0.1-0.24 M alkaline earth salt, pH 7-7.4; and (D) eluting (I) with a solution of at least 1.3 M alkali salt or 0.27 M alkaline earth salt, pH 7-7.4.
IPC 8 full level
C12N 15/10 (2006.01)
CPC (source: EP US)
A61P 43/00 (2018.01 - EP); C12N 15/101 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
EP 1559783 A1 20050803; AU 2005209389 A1 20050811; CN 1914319 A 20070214; CN 1914319 B 20100609; EP 1713910 A1 20061025; JP 2007519407 A 20070719; US 2007275920 A1 20071129; WO 2005073376 A1 20050811
DOCDB simple family (application)
EP 04001864 A 20040129; AU 2005209389 A 20050125; CN 200580003764 A 20050125; EP 05706998 A 20050125; EP 2005000693 W 20050125; JP 2006550069 A 20050125; US 58789207 A 20070618