EP 1723254 A1 20061122 - QUALITY ENSUREMENT SYSTEM FOR DETECTING MICROORGANISMS
Title (en)
QUALITY ENSUREMENT SYSTEM FOR DETECTING MICROORGANISMS
Title (de)
QUALITÄTSSICHERUNGSSYSTEM ZUM NACHWEIS VON MIKROORGANISMEN
Title (fr)
SYSTEME D'ASSURANCE QUALITE PERMETTANT DE DECELER DES MICRO-ORGANISMES
Publication
Application
Priority
- EP 2005002209 W 20050303
- DE 102004011822 A 20040311
Abstract (en)
[origin: WO2005087944A1] The invention relates to a quality ensurement system for detecting reproducible microorganisms, comprising a) a system for enriching microorganisms in a sample in an over-night culture corresponding to 8-24 hours of cultivation in standard conditions according to international pharmaceutical statutes, food statutes and cosmetic laws or according to usual market indirect methods; b) a kit for detecting living, damaged or dead microorganisms in filterable and/or non-filterable samples or products, containing i) at least one reagent containing an inductor and a fluorescent reagent which leads to the formation of a specific enzyme in the case of living cells, released a fluorescent dye by means of reaction with a specific fluorescent dye reagent, said dye being detectable; ii) at least one nucleic acid probe for detecting microorganisms by in-situ hybridization, wherein the nucleic acid probe is bound to a fluorescent marker,wherein a detection limit < 10 CFU/g of is obtained for reproducible microorganisms. The invention also relates to the use of the inventive quality ensurement system and to a method for detecting living, damaged or dead microorganisms in filterable and/or non-filterable samples or products using the inventive quality ensurement system.
IPC 8 full level
C12Q 1/04 (2006.01); C12Q 1/18 (2006.01); C12Q 1/22 (2006.01); C12Q 1/25 (2006.01); C12Q 1/44 (2006.01); C12Q 1/54 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/04 (2013.01 - EP US); C12Q 1/6841 (2013.01 - EP US); C12Q 1/689 (2013.01 - EP US)
Citation (search report)
See references of WO 2005087944A1
Designated contracting state (EPC)
DE ES FR GB IT TR
DOCDB simple family (publication)
WO 2005087944 A1 20050922; BR PI0508620 A 20070814; CN 1934268 A 20070321; DE 102004011822 A1 20050929; EP 1723254 A1 20061122; JP 2007527723 A 20071004; US 2007243532 A1 20071018
DOCDB simple family (application)
EP 2005002209 W 20050303; BR PI0508620 A 20050303; CN 200580007790 A 20050303; DE 102004011822 A 20040311; EP 05715673 A 20050303; JP 2007502237 A 20050303; US 59877605 A 20050303