EP 1735471 A2 20061227 - METHOD OF EXAMINING THE FUNCTIONAL GENIC VARIABILITY OF HIV AND KIT FOR IMPLEMENTING SAME
Title (en)
METHOD OF EXAMINING THE FUNCTIONAL GENIC VARIABILITY OF HIV AND KIT FOR IMPLEMENTING SAME
Title (de)
VERFAHREN ZUR PRÜFUNG DER FUNKTIONELLEN GENVARIABILITÄT DES HIV UND KIT FÜR DESSEN IMPLEMENTIERUNG
Title (fr)
METHODE D"ETUDE DE LA VARIABILITE GENIQUE ET FONCTIONNELLE DU VIH ET KIT POUR SA MISE EN OEUVRE
Publication
Application
Priority
- FR 2005000917 W 20050415
- FR 0404039 A 20040416
Abstract (en)
[origin: WO2005108606A2] The invention relates to a method of analysing a sample that may contain HIV virus. The inventive method comprises the following steps: (a) extraction of the viral RNA; (b) inverse transcription of the RNA obtained in step (a) and amplification of same with a first primer pair, thereby enabling the production of an amplified reverse transcription product comprising all or part of at least two successive genes of the genome of a HIV virus; (c) sequencing of the amplified reverse transcription product; (d) amplification of the amplified reverse transcription product obtained in step (b) with a second primer pair; (e) homologous recombination of the amplification product prepared in step (d) with a vector; (f) functional analysis of viral proteins encoded by all or part of at least two genes; and (g) measurement of the replicative capacity of the recombinant viruses obtained in step (e).
IPC 8 full level
C12Q 1/70 (2006.01)
CPC (source: EP US)
C12Q 1/703 (2013.01 - EP US)
Citation (search report)
See references of WO 2005108606A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA HR LV MK YU
DOCDB simple family (publication)
FR 2869045 A1 20051021; CA 2563394 A1 20051117; CN 1969048 A 20070523; EP 1735471 A2 20061227; US 2006292553 A1 20061228; WO 2005108606 A2 20051117; WO 2005108606 A3 20060817
DOCDB simple family (application)
FR 0404039 A 20040416; CA 2563394 A 20050415; CN 200580019710 A 20050415; EP 05757257 A 20050415; FR 2005000917 W 20050415; US 7397205 A 20050307