EP 1737978 A1 20070103 - NUCLEIC ACID SEQUENCING
Title (en)
NUCLEIC ACID SEQUENCING
Title (de)
NUKLEINSÄURESEQUENZIERUNG
Title (fr)
SEQUENCAGE D'ACIDE NUCLEIQUE
Publication
Application
Priority
- IB 2005000771 W 20050324
- GB 0406863 A 20040326
Abstract (en)
[origin: WO2005093101A1] A method for determining a target nucleic acid sequence, wherein the target nucleic acid sequence is comprised in a preparation comprising a non-target nucleic acid sequence, the target nucleic acid sequence and the non-target nucleic acid sequence each having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, the method comprising: (a) contacting the preparation with a blocking oligonucleotide complementary to at least a portion of the first region of dissimilar sequence of the non-target nucleic acid sequence, under conditions to hybridise the blocking oligonucleotide thereto; (b) contacting the preparation with a sequencing primer complementary to at least a portion of the first region of common sequence, under conditions to hybridise the primer to the target nucleic acid sequence; and (c) subjecting the preparation to a sequencing reaction, such that the sequencing reaction proceeds into the second region of dissimilar sequence of the target nucleic acid sequence, thereby determining at least the second region of dissimilar sequence of the target nucleic acid sequence; and wherein the blocking oligonucleotide blocks the sequencing reaction at least from proceeding into the second region of dissimilar sequence of the non-target nucleic acid sequence.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/6813 (2013.01); C12Q 1/6858 (2013.01); C12Q 1/6869 (2013.01)
Citation (search report)
See references of WO 2005093101A1
Citation (examination)
- HANNA M M ET AL: "RNA-protein crosslinking with photoreactive nucleotide analogs", 1 January 1999, RNA-PROTEIN INTERACTION PROTOCOLS, METHODS IN MOLECULAR BIOLOGY, HUMANA PRESS, TOTOWA, NJ, PAGE(S) 21 - 33, ISBN: 978-0-89603-568-3, XP008105674
- STUMP W T ET AL: "Crosslinking of an iodo-uridine-RNA hairpin to a single site on the human U1A N-terminal RNA binding domain.", RNA (NEW YORK, N.Y.) MAR 1995 LNKD- PUBMED:7489489, vol. 1, no. 1, March 1995 (1995-03-01), pages 55 - 63, ISSN: 1355-8382
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005093101 A1 20051006; EP 1737978 A1 20070103; GB 0406863 D0 20040428; JP 2007530026 A 20071101
DOCDB simple family (application)
IB 2005000771 W 20050324; EP 05718267 A 20050324; GB 0406863 A 20040326; JP 2007504507 A 20050324