EP 1739188 B1 20110928 - METHOD OF MEASURING ACTIVITY OF HOMOCYSTEINE THIOLACTONE HYDROLASE
Title (en)
METHOD OF MEASURING ACTIVITY OF HOMOCYSTEINE THIOLACTONE HYDROLASE
Title (de)
VERFAHREN ZUR MESSUNG DER AKTIVITÄT VON HOMOCYSTEIN-THIOLACTON-HYDROLASE
Title (fr)
PROCÉDÉ DE MESURE DE L'ACTIVITÉ DE L'HOMOCYSTÉINE THIOLACTONE HYDROLASE
Publication
Application
Priority
- JP 2005007050 W 20050412
- JP 2004122926 A 20040419
Abstract (en)
[origin: EP1739188A1] Disclosed is a method for measurement of the activity of the homocysteinethiolactone hydrolyzing enzyme in a sample, which method comprises mixing and reacting ³-thiobutyrolactone and the sample in the presence of a cholinesterase inhibitor, and measuring 4-mercaptobutyric acid thereby produced. The method is safe and easy in operation, for it does not use a radioactive substrate, and is free of the influence of other compounds occurring in a sample from the body.
IPC 8 full level
C12Q 1/34 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP US)
C12Q 1/34 (2013.01 - EP US)
Designated contracting state (EPC)
DE FR GB IT
DOCDB simple family (publication)
EP 1739188 A1 20070103; EP 1739188 A4 20090415; EP 1739188 B1 20110928; JP 2005304329 A 20051104; JP 4729264 B2 20110720; US 2007243571 A1 20071018; US 7534581 B2 20090519; WO 2005103286 A1 20051103
DOCDB simple family (application)
EP 05730497 A 20050412; JP 2004122926 A 20040419; JP 2005007050 W 20050412; US 57872905 A 20050412