EP 1747456 A1 20070131 - FRONTAL AFFINITY CHROMATOGRAPHY/MALDI TANDEM MASS SPECTROMETRY
Title (en)
FRONTAL AFFINITY CHROMATOGRAPHY/MALDI TANDEM MASS SPECTROMETRY
Title (de)
FRONTALAFFINITÄTSCHROMATOGRAPHIE/MALDI-TANDEM-MASSENSPEKTROMETRIE
Title (fr)
CHROMATOGRAPHIE D'AFFINITE FRONTALE/SPECTROMETRIE DE MASSE EN TANDEM MALDI
Publication
Application
Priority
- CA 2005000790 W 20050524
- US 57300904 P 20040521
Abstract (en)
[origin: WO2005114170A1] Sol-gel derived monolithic silica columns containing entrapped dihydrofolate reductase were used for frontal affinity chromatography of small molecule mixtures. The output from the column combined with a second stream containing the matrix molecule (HCCA) and was directly deposited onto a conventional MALDI plate that moved relative to the column via a computer controlled x-y stage, creating a semi-permanent record of the FAC run. The use of MALDI MS allowed for a decoupling of the FAC and MS methods allowing significantly higher ionic strength buffers to be used for FAC studies, which allowed for better retention of protein activity over multiple runs.
IPC 8 full level
G01N 30/72 (2006.01); H01J 49/16 (2006.01); G01N 30/02 (2006.01)
CPC (source: EP)
G01N 30/7286 (2013.01); H01J 49/164 (2013.01); G01N 30/02 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005114170 A1 20051201; CA 2566815 A1 20051201; EP 1747456 A1 20070131; EP 1747456 A4 20090513; JP 2007538235 A 20071227
DOCDB simple family (application)
CA 2005000790 W 20050524; CA 2566815 A 20050524; EP 05748644 A 20050524; JP 2007516919 A 20050524