Global Patent Index - EP 1747456 A4

EP 1747456 A4 20090513 - FRONTAL AFFINITY CHROMATOGRAPHY/MALDI TANDEM MASS SPECTROMETRY

Title (en)

FRONTAL AFFINITY CHROMATOGRAPHY/MALDI TANDEM MASS SPECTROMETRY

Title (de)

FRONTALAFFINITÄTSCHROMATOGRAPHIE/MALDI-TANDEM-MASSENSPEKTROMETRIE

Title (fr)

CHROMATOGRAPHIE D'AFFINITE FRONTALE/SPECTROMETRIE DE MASSE EN TANDEM MALDI

Publication

EP 1747456 A4 20090513 (EN)

Application

EP 05748644 A 20050524

Priority

  • CA 2005000790 W 20050524
  • US 57300904 P 20040521

Abstract (en)

[origin: WO2005114170A1] Sol-gel derived monolithic silica columns containing entrapped dihydrofolate reductase were used for frontal affinity chromatography of small molecule mixtures. The output from the column combined with a second stream containing the matrix molecule (HCCA) and was directly deposited onto a conventional MALDI plate that moved relative to the column via a computer controlled x-y stage, creating a semi-permanent record of the FAC run. The use of MALDI MS allowed for a decoupling of the FAC and MS methods allowing significantly higher ionic strength buffers to be used for FAC studies, which allowed for better retention of protein activity over multiple runs.

IPC 8 full level

G01N 30/72 (2006.01); H01J 49/16 (2006.01); G01N 30/02 (2006.01)

CPC (source: EP)

G01N 30/7286 (2013.01); H01J 49/164 (2013.01); G01N 30/02 (2013.01)

C-Set (source: EP)

G01N 30/02 + B01D 15/3804

Citation (search report)

  • [X] WO 9950669 A1 19991007 - SYNSORB BIOTECH INC [CA], et al
  • [X] US 2003044848 A1 20030306 - RUSH JOHN [US], et al
  • [X] US 2002195555 A1 20021226 - WEINBERGER SCOT R [US], et al
  • [A] US 2002175278 A1 20021128 - WHITEHOUSE CRAIG M [US]
  • [X] ZHANG B ET AL: "COMBINING LIQUID CHROMATOGRAPHY WITH MALDI MASS SPECTROMETRY USING A HEATED DROPLET INTERFACE", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 76, no. 4, 15 February 2004 (2004-02-15), pages 992 - 1001, XP001047414, ISSN: 0003-2700
  • [X] SCHRIEMER D C ET AL: "COMBINING AVIDIN-BIOTIN CHEMISTRY WITH MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 68, no. 19, 1 October 1996 (1996-10-01), pages 3382 - 3387, XP009081922, ISSN: 0003-2700
  • [XY] RASKA CHRISTINA S ET AL: "Direct MALDI-MS/MS of phosphopeptides affinity-bound to immobilized metal ion affinity chromatography beads", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 74, no. 14, 15 July 2002 (2002-07-15), pages 3429 - 3433, XP002416437, ISSN: 0003-2700
  • [XY] ZHANG B ET AL: "Frontal affinity chromatography coupled to mass spectrometry for screening mixtures of enzyme inhibitors", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC, NEW YORK, vol. 299, no. 2, 15 December 2001 (2001-12-15), pages 173 - 182, XP002219168, ISSN: 0003-2697
  • [YA] HODGSON RICHARD J ET AL: "Protein-doped monolithic silica columns for capillary liquid chromatography prepared by the sol-gel method: applications to frontal affinity chromatography.", ANALYTICAL CHEMISTRY 15 MAY 2004, vol. 76, no. 10, 15 May 2004 (2004-05-15), pages 2780 - 2790, XP002521731, ISSN: 0003-2700
  • [A] ZHANG H ET AL: "CAPILLARY ELECTROPHORESIS COMBINED WITH MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY; CONTINUOUS SAMPLE DEPOSITION ON A MATRIX-PRECOATED MEMBRANE TARGET", JOURNAL OF MASS SPECTROMETRY, WILEY, CHICHESTER, GB, vol. 31, 1 January 1996 (1996-01-01), pages 1039 - 1046, XP002910712, ISSN: 1076-5174
  • See references of WO 2005114170A1

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR

DOCDB simple family (publication)

WO 2005114170 A1 20051201; CA 2566815 A1 20051201; EP 1747456 A1 20070131; EP 1747456 A4 20090513; JP 2007538235 A 20071227

DOCDB simple family (application)

CA 2005000790 W 20050524; CA 2566815 A 20050524; EP 05748644 A 20050524; JP 2007516919 A 20050524