EP 1761646 A1 20070314 - METHOD FOR QUANTIFYING METHYLATED DNA
Title (en)
METHOD FOR QUANTIFYING METHYLATED DNA
Title (de)
VERFAHREN ZUR QUANTIFIZIERUNG METHYLIERTER DNA
Title (fr)
PROCEDE DE QUANTIFICATION D'ADN METHYLE
Publication
Application
Priority
- DE 2005001109 W 20050615
- DE 102004029700 A 20040615
Abstract (en)
[origin: WO2005123941A1] The inventive method makes it possible to quantify methylated DNA by combining the restricted digestion and real-time PCR. For this purpose, the inventive method consists in isolating the examined DNA from a biological sample, in reacting the isolated AND with a methylation specific restriction enzyme, in amplifying by means of a real-time PCR, wherein the amplified products are formed only when the DNA is precut-off and, afterwards, in calculated the methylated and unmethylated DNA proportion in the initial sample with the aid of a reference measurement. Said method is particularly suitable for diagnosis and prognosis cancer and other diseases associated with a methylation state modification and for predicting the drug effects.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6827 (2013.01 - EP US)
Citation (search report)
See references of WO 2005123941A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005123941 A1 20051229; DE 102004029700 A1 20060209; EP 1761646 A1 20070314; US 2009004646 A1 20090101
DOCDB simple family (application)
DE 2005001109 W 20050615; DE 102004029700 A 20040615; EP 05756101 A 20050615; US 62974305 A 20050615