EP 1789572 A2 20070530 - MICROORGANISM DETECTION USING BACTERIOPHAGE AMPLIFICATION
Title (en)
MICROORGANISM DETECTION USING BACTERIOPHAGE AMPLIFICATION
Title (de)
NACHWEIS VON MIKROORGANISMEN MITTELS BAKTERIOPHAGENAMPLIFIKATION
Title (fr)
DETECTION DE MICRO-ORGANISMES AU MOYEN D'UNE AMPLIFICATION BACTERIOPHAGE
Publication
Application
Priority
- US 2005020248 W 20050607
- US 57784504 P 20040607
- US 60794104 P 20040907
Abstract (en)
[origin: WO2006083292A2] A method of detecting the presence or absence of a target microorganism in a sample to be tested comprising: combining with the sample, bacteriophage capable of infecting the target microorganism to create a bacteriophage exposed sample; providing conditions to the bacteriophage exposed sample sufficient to allow the bacteriophage to infect the target microorganism to create injected bacteriophage nucleic acid, additional bacteriophage nucleic acid, intermediate bacteriophage protein, or additional bacteriophage protein; and assaying the nucleic acid or protein to determine the presence or absence of the target microorganism. The microorganism is preferably dissociated or lysed prior to the completion of the bacteriophage replication process. The sample is an insitu sample and the microorganism is isolated from the insitu matrix. The assaying comprises comparing the test nucleic acid or protein level to a reference level.
IPC 8 full level
C12Q 1/04 (2006.01); C12Q 1/70 (2006.01); G01N 33/569 (2006.01)
CPC (source: EP)
C12Q 1/04 (2013.01); C12Q 1/689 (2013.01); C12Q 1/70 (2013.01); G01N 33/56911 (2013.01)
Citation (search report)
See references of WO 2006083292A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA HR LV MK YU
DOCDB simple family (publication)
WO 2006083292 A2 20060810; WO 2006083292 A3 20070426; EP 1789572 A2 20070530
DOCDB simple family (application)
US 2005020248 W 20050607; EP 05856796 A 20050607