EP 1794298 A2 20070613 - PRODUCTION OF MERSACIDIN AND ITS VARIANTS IN SIGH AND/OR MRSA NEGATIVE BACILLUS HOST CELLS
Title (en)
PRODUCTION OF MERSACIDIN AND ITS VARIANTS IN SIGH AND/OR MRSA NEGATIVE BACILLUS HOST CELLS
Title (de)
PRODUKTION VON MERSACIDIN UND SEINEN VARIANTEN IN SIGH- UND/ODER MRSA-NEGATIVEN BACILLUS-WIRTSZELLEN
Title (fr)
PRODUCTION DE MERSACIDINE ET SES VARIANTS DANS DES CELLULES HOTES NEGATIVES DE BACILLUS SIGH ET/OU MRSA
Publication
Application
Priority
- GB 2005001055 W 20050321
- GB 0406870 A 20040326
Abstract (en)
[origin: WO2005093069A2] The present invention relates to methods and products for improved production of the lantibiotic mersacidin. There is provided a method of producing a mersacidin variant which comprises introducing into a cell which is a negative MrsA host cell an expression vector encoding said variant, and recovering said variant from the cell culture. Preferred variants include the novel variants mersacidin F3W, mersacidin G8A and mersacidin F3W G8A. Also provided is a Bacillus cell which is a SigH deficient strain of Bacillus sp. HIL Y-85,54728 (NCIMB Accession Number NCIMB 41211); this cell may also be used in the production of mersacidin and its variants. Further aspects of the invention include a DNA cassette which comprises a nucleotide sequence encoding the mersacidin mrsA propeptide, and a method to transform a Bacillus HIL host cell with plasmid, which method includes the step of electroporation.
IPC 8 full level
C12N 15/31 (2006.01); C07K 7/56 (2006.01); C07K 14/32 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/75 (2006.01); C12P 21/02 (2006.01); C12P 21/04 (2006.01); A61K 38/00 (2006.01); C12R 1/07 (2006.01)
CPC (source: EP)
C07K 14/32 (2013.01); A61K 38/00 (2013.01)
Citation (search report)
See references of WO 2005093069A2
Citation (examination)
WO 2007036706 A1 20070405 - NOVACTA BIOSYSTEMS LTD [GB], et al
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2005093069 A2 20051006; WO 2005093069 A3 20051208; AU 2005225595 A1 20051006; EP 1794298 A2 20070613; GB 0406870 D0 20040428
DOCDB simple family (application)
GB 2005001055 W 20050321; AU 2005225595 A 20050321; EP 05729379 A 20050321; GB 0406870 A 20040326