EP 1794320 A1 20070613 - METHOD FOR GENERATING TRANSCRIPTS
Title (en)
METHOD FOR GENERATING TRANSCRIPTS
Title (de)
VERFAHREN ZUR ERZEUGUNG VON TRANSKRIPTEN
Title (fr)
MÉTHODE POUR GÉNÉRER DES TRANSCRITS
Publication
Application
Priority
- FR 2005050775 W 20050923
- FR 0452228 A 20041001
Abstract (en)
[origin: US2007260053A1] The present invention relates to a method for generating transcripts from: at least one RNA sequence to be amplified comprising a "primer" region and a region of interest, and an amplification primer comprising a promoter region, and a region capable of hybridizing to said "primer" region of the RNA sequence to be amplified, said method being carried out at constant temperature, and comprising the following steps: a) said primer is hybridized with the RNA to be amplified, b) the primer is extended by means of a reverse transcriptase enzymatic activity in order to generate a complementary deoxyribonucleic acid (cDNA) sequence of the RNA to be amplified, c) the RNA to be amplified, hybridized to said cDNA, is cleaved by means of an enzyme that has a ribonuclease H activity, so as to obtain fragments of RNA to be amplified, hybridized to said cDNA, d) the ends of said fragments of RNA to be amplified are extended by means of a reverse transcriptase and strand displacement enzyme, so as to obtain RNA-DNA/DNA hybrids, e) RNA transcripts are obtained from the RNA-DNA/DNA hybrids formed in step d), by means of an enzyme that has an RNA polymerase activity.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6865 (2013.01 - EP US)
C-Set (source: EP US)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
FR 2876116 A1 20060407; FR 2876116 B1 20070406; AT E397675 T1 20080615; CN 101044250 A 20070926; DE 602005007366 D1 20080717; EP 1794320 A1 20070613; EP 1794320 B1 20080604; ES 2308570 T3 20081201; JP 2008514220 A 20080508; US 2007260053 A1 20071108; WO 2006054003 A1 20060526
DOCDB simple family (application)
FR 0452228 A 20041001; AT 05805383 T 20050923; CN 200580036116 A 20050923; DE 602005007366 T 20050923; EP 05805383 A 20050923; ES 05805383 T 20050923; FR 2005050775 W 20050923; JP 2007534058 A 20050923; US 66095205 A 20050923