Global Patent Index - EP 1815022 A4

EP 1815022 A4 20090325 - METHOD OF DETERMINING A CELLULAR RESPONSE TO A BIOLOGICAL AGENT

Title (en)

METHOD OF DETERMINING A CELLULAR RESPONSE TO A BIOLOGICAL AGENT

Title (de)

VERFAHREN ZUR BESTIMMUNG DER ZELLREAKTION AUF EINEN BIOLOGISCHEN WIRKSTOFF

Title (fr)

MÉTHODE DE DÉTERMINATION D'UNE RÉPONSE CELLULAIRE À UN AGENT BIOLOGIQUE

Publication

EP 1815022 A4 20090325 (EN)

Application

EP 05849672 A 20051121

Priority

  • US 2005042407 W 20051121
  • US 63032004 P 20041122
  • US 67816505 P 20050504
  • US 28348105 A 20051118
  • US 28348205 A 20051118
  • US 28348305 A 20051118
  • US 28348405 A 20051118

Abstract (en)

[origin: CA2587786A1] A reverse transfection apparatus can be used for introducing siRNA into a ce ll to effect gene silencing. Such an apparatus can include a well plate having a well configured for transfecting cells. The well can include a substantially dry gene silencing composition that has at least a first siRNA which silence s a first target gene. The gene silencing composition can be configured such that the at least first siRNA is capable of being solubilized or suspended i n an aqueous medium in an amount sufficient for transfecting cells in the well . Additionally, the at least first siRNA can include a modification or a conjugate. The reverse transfection apparatus can be provided as a kit or system that additionally includes cells, polynucleotide carriers, reverse transfection reagents, and the like.

IPC 8 full level

C07H 19/00 (2006.01); A61K 31/70 (2006.01); C12N 15/11 (2006.01); C12N 15/87 (2006.01); C12Q 1/68 (2006.01); G01N 33/50 (2006.01)

CPC (source: EP KR)

C12M 1/00 (2013.01 - KR); C12M 1/18 (2013.01 - KR); C12M 1/26 (2013.01 - KR); C12Q 1/6809 (2013.01 - EP); G01N 33/502 (2013.01 - EP)

C-Set (source: EP)

C12Q 1/6809 + C12Q 2545/113 + C12Q 2545/101 + C12Q 2525/207

Citation (search report)

  • [XY] US 6294350 B1 20010925 - PETERSON THERESA C [CA]
  • [Y] WO 2004078946 A2 20040916 - UNIV LELAND STANFORD JUNIOR [US], et al
  • [Y] WO 2004090105 A2 20041021 - DHARMACON INC [US], et al
  • [PX] WO 2005097992 A2 20051020 - DHARMACON INC [US], et al
  • [XY] VANHECKE D ET AL: "High-throughput gene silencing using cell arrays", ONCOGENE, NATURE PUBLISHING GROUP, GB BASINGSTOKE, HANTS, vol. 23, no. 51, 1 November 2004 (2004-11-01), pages 8353 - 8358, XP002397165, ISSN: 0950-9232
  • [XY] AZA-BLANC P ET AL: "Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening", MOLECULAR CELL, CELL PRESS, CAMBRIDGE, MA, US, vol. 12, no. 3, 1 September 2003 (2003-09-01), pages 627 - 637, XP002350184, ISSN: 1097-2765
  • [X] MINAKUCHI YOSHIKO ET AL: "Atelocollagen-mediated synthetic small interfering RNA delivery for effective gene silencing in vitro and in vivo.", NUCLEIC ACIDS RESEARCH 2004, vol. 3132, no. 13, 22 July 2004 (2004-07-22), pages e109, XP002513223, ISSN: 1362-4962
  • [X] HONMA K ET AL: "Atelocollagen-based gene transfer in cells allows high-throughput screening of gene functions.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 21 DEC 2001, vol. 289, no. 5, 21 December 2001 (2001-12-21), pages 1075 - 1081, XP002957375, ISSN: 0006-291X
  • [A] ZHOU N ET AL: "Inhibition of HIV-1 fusion with small interfering RNAs targeting the chemokine coreceptor CXCR4.", GENE THERAPY DEC 2004, vol. 11, no. 23, 12 August 2004 (2004-08-12), pages 1703 - 1712, XP002513225, ISSN: 0969-7128
  • [A] "Recommendations for successful siRNA Library screens", AMBION CATALOGUE,, vol. 11, no. 6, 1 November 2004 (2004-11-01), pages 15 - 16, XP002513243
  • See references of WO 2006058048A2

Citation (examination)

  • DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 15 December 2001 (2001-12-15), HOU MING-HON ET AL: "Effects of polyamines on the thermal stability and formation kinetics of DNA duplexes with abnormal structure", Database accession no. PREV200200160484 & NUCLEIC ACIDS RESEARCH, vol. 29, no. 24, 15 December 2001 (2001-12-15), pages 5121 - 5128, ISSN: 0305-1048
  • LEE CHAN YONG ET AL: "Inhibitory effect of spermine on the susceptibility of RNA for RNase A", J OF THE KOREAN CHEM SOC, vol. 29, no. 6, 1985, pages 646 - 650

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

DOCDB simple family (publication)

AT E519772 T1 20110815; AU 2005322468 A1 20060706; AU 2005322468 A2 20060706; AU 2005322468 B2 20110310; CA 2587786 A1 20060706; CA 2587786 C 20171205; CN 101107352 A 20080116; CN 101107352 B 20130320; EP 1814895 A2 20070808; EP 1814895 A4 20080528; EP 1814895 B1 20110810; EP 1814994 A2 20070808; EP 1814994 A4 20100714; EP 1815022 A2 20070808; EP 1815022 A4 20090325; JP 2008532480 A 20080821; KR 101277646 B1 20130625; KR 20070118585 A 20071217

DOCDB simple family (application)

AT 05852038 T 20051121; AU 2005322468 A 20051121; CA 2587786 A 20051121; CN 200580047054 A 20051121; EP 05849672 A 20051121; EP 05852038 A 20051121; EP 05857003 A 20051121; JP 2007543423 A 20051121; KR 20077013989 A 20051121