EP 1819815 A2 20070822 - METHOD OF ACCELERATING SOMATIC MUTATIONS AND USE THEREOF IN PROTEOMICS
Title (en)
METHOD OF ACCELERATING SOMATIC MUTATIONS AND USE THEREOF IN PROTEOMICS
Title (de)
VERFAHREN ZUR BESCHLEUNIGUNG VON SOMATISCHEN MUTATIONEN UND VERWENDUNG DAVON IN DER PROTEOMIK
Title (fr)
PROCEDE POUR L'ACCELERATION DES MUTATIONS SOMATIQUES ET SON APPLICATION EN PROTEOMIQUE
Publication
Application
Priority
- FR 2005002260 W 20050912
- FR 0409605 A 20040910
Abstract (en)
[origin: WO2006030126A2] The invention relates to a method of accelerating the induction of somatic mutations in vitro. The inventive method comprises the expression of at least one cDNA expressing a modified version of the AID gene in the cells to be mutated, in culture conditions and a medium that are suited thereto, said modified version resulting from an AID gene in which the three hydrophobic amino acids, leul89, phe193 and leul96, have been replaced by means of alanine mutation in each case. The invention can be used to induce mutations in Burkitt's lymphoma BL2. The invention can also be used to induce mutations in the immunoglobulin genes of immortalised antibody-producing cells, such as mouse hybridoma cells, human hybridoma cells or human B-cell lines immortalised by the Epstein-Barr virus (EBV).
IPC 8 full level
C12N 9/78 (2006.01); C12N 15/55 (2006.01); C12Q 1/68 (2006.01); G01N 33/50 (2006.01)
CPC (source: EP US)
C12N 9/78 (2013.01 - EP US); C12N 15/102 (2013.01 - EP US)
Citation (search report)
See references of WO 2006030126A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
FR 2875239 A1 20060317; FR 2875239 B1 20070720; AU 2005284022 A1 20060323; CA 2580011 A1 20060323; EP 1819815 A2 20070822; JP 2008512107 A 20080424; US 2009209036 A1 20090820; WO 2006030126 A2 20060323; WO 2006030126 A3 20060526
DOCDB simple family (application)
FR 0409605 A 20040910; AU 2005284022 A 20050912; CA 2580011 A 20050912; EP 05802633 A 20050912; FR 2005002260 W 20050912; JP 2007530744 A 20050912; US 57499705 A 20050912