EP 1833846 A2 20070919 - METHOD FOR PRODUCTION OF A THERAPEUTICALLY PURE GRANULOCYTE MACROPHAGE - COLONY STIMULATING FACTOR
Title (en)
METHOD FOR PRODUCTION OF A THERAPEUTICALLY PURE GRANULOCYTE MACROPHAGE - COLONY STIMULATING FACTOR
Title (de)
VERFAHREN ZUR HERSTELLUNG EINES THERAPEUTISCH REINEN GRANULOZYTENMACROPHAGEN-KOLONIESTIMULIERENDEN FAKTORS
Title (fr)
PROCEDE POUR LA PRODUCTION D'UN FACTEUR STIMULANT LES COLONIES DE GRANULOCYTES-MACROPHAGES
Publication
Application
Priority
- IN 2005000088 W 20050318
- IN 1343CH2004 A 20041209
Abstract (en)
[origin: WO2006061851A2] A simple and scaleable process is provided for obtaining recombinant human GM CSF expressed in microbial cells, secreting mature GM-CSF, culturing these cells and purifying the protein in good yield and of a therapeutic grade quality. The steps include cloning, fermentation and purification of the protein. The purified protein is got by a process that involves lysing the microorganism, extraction of the processed and unprocessed forms of GM-CSF from the membrane pellet and selectively purifying the processed form of the protein from the unprocessed form by a two-step chromatography procedure. The purity of the processed form of GM-CSF (without signal peptide) obtained has endotoxin units, host cell proteins and host cell DNA within the specified compendial limits for therapeutic proteins.
IPC 8 full level
C12N 15/00 (2006.01); C07K 5/00 (2006.01); C12P 21/00 (2006.01)
CPC (source: EP)
C07K 14/535 (2013.01)
Citation (search report)
See references of WO 2006061851A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA HR LV MK YU
DOCDB simple family (publication)
WO 2006061851 A2 20060615; WO 2006061851 A3 20090423; AP 2007004049 A0 20070630; EP 1833846 A2 20070919
DOCDB simple family (application)
IN 2005000088 W 20050318; AP 2007004049 A 20050318; EP 05732861 A 20050318