EP 1833994 A2 20070919 - PRIMER GENERATION ROLLING CIRCLE AMPLIFICATION
Title (en)
PRIMER GENERATION ROLLING CIRCLE AMPLIFICATION
Title (de)
ROLLING-CIRCLE-VERSTÄRKUNG EINER PRIMER-ERZEUGUNG
Title (fr)
AMPLIFICATION EN CERCLE ROULANT POUR LA PRODUCTION D'AMORCES
Publication
Application
Priority
- US 2006000086 W 20060104
- US 64125505 P 20050104
- US 69934005 P 20050714
Abstract (en)
[origin: WO2006074162A2] A method of amplifying a nucleic acid is provided which comprises: generating a first nucleic acid primer from a first nucleic acid sequence; combining the first nucleic acid primer with a first polymerase and a first circular nucleic acid probe, wherein the first circular nucleic acid probe contains at least one antisense sequence to a second nucleic acid sequence and at least one antisense sequence to the first nucleic acid primer; producing at least one repeat of a sequence copy of the first circular nucleic acid probe by rolling circle amplification using the first polymerase, wherein the sequence copy contains at least the second nucleic acid sequence; generating a second nucleic acid primer from the second nucleic acid sequence; combining the second nucleic acid primer with a second polymerase and a second circular nucleic acid probe, where the second circular nucleic acid probe contains at least one antisense sequence to the second nucleic acid primer; and producing at least one repeat of a sequence copy of the second circular nucleic acid probe by rolling circle amplification using the second polymerase. The method may be employed to detect molecules of interest such as nucleic acid sequences, DNA methylation, single nucleotide polymorphisms (SNP), proteins and posttranslational modifications. Furthermore, a ribbon probe is provided that comprises a circular nucleic acid probe and a nucleic acid lock probe, wherein: the nucleic acid lock probe contains at least a cleavable linker, and the circular nucleic acid probe and the lock probe are unable to dissociate without cleaving the cleavable linker.
IPC 8 full level
C07H 21/04 (2006.01); C12P 19/34 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP US)
C12P 19/34 (2013.01 - EP US); C12Q 1/6844 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2006074162 A2 20060713; WO 2006074162 A3 20060831; EP 1833994 A2 20070919; EP 1833994 A4 20090902; EP 2319940 A1 20110511; JP 2008526228 A 20080724; JP 5068175 B2 20121107; US 2009233277 A1 20090917; US 8916351 B2 20141223
DOCDB simple family (application)
US 2006000086 W 20060104; EP 06717311 A 20060104; EP 10195226 A 20060104; JP 2007550421 A 20060104; US 81314206 A 20060104