Global Patent Index - EP 1841882 A1

EP 1841882 A1 20071010 - PROTEIN PHOSPHORYLATION IN EGFR-SIGNALING PATHWAYS

Title (en)

PROTEIN PHOSPHORYLATION IN EGFR-SIGNALING PATHWAYS

Title (de)

PROTEINPHOSPHORYLIERUNG IN EGFR-SIGNALWEGEN

Title (fr)

PHOSPHORYLATION DES PROTEINES SUIVANT DES VOIES CONTROLEES PAR LES EGFR

Publication

EP 1841882 A1 20071010 (EN)

Application

EP 04815061 A 20041221

Priority

US 2004042940 W 20041221

Abstract (en)

[origin: WO2006068640A1] The invention discloses 168 novel phosphorylation sites identified in signal transduction proteins and pathways downstream of, and including, EGFR kinase, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: Actin Binding proteins, Adaptor/Scaffold proteins, Calcium-Binding Proteins, Cell Cycle Regulation proteins, Cytoskeletal proteins, DNA Binding and Replication Proteins, GTPase Activating proteins, Guanine Nucleotide Exchange Factor proteins, Lipid Kinases, Receptor Tyrosine Kinases, Receptor Tyrosine Kinase ligands, Protein Kinases, Receptor and Protein Phosphatases, Transcription Factor proteins, Tumor Suppressor proteins, and Vesicle proteins.

IPC 8 full level

C12Q 1/48 (2006.01); C07K 7/00 (2006.01); C07K 16/00 (2006.01); G01N 33/68 (2006.01)

CPC (source: EP)

C07K 16/2866 (2013.01); C07K 16/2869 (2013.01); C07K 16/32 (2013.01); C07K 16/40 (2013.01); C07K 16/44 (2013.01); G01N 33/6872 (2013.01); G01N 33/74 (2013.01); C07K 2317/34 (2013.01); G01N 2333/4706 (2013.01); G01N 2500/04 (2013.01)

Citation (search report)

  • [A] US 2003044848 A1 20030306 - RUSH JOHN [US], et al
  • [A] WO 03016861 A2 20030227 - HARVARD COLLEGE [US], et al & PRIGENT S A ET AL: "Identification of c-erbB-3 binding sites for phosphatidylinositol 3'-kinase and SHC using an EGF receptor/c-erbB-3 chimera", EMBO J, vol. 13, no. 12, 1994, pages 2831 - 2841, XP002467288 & KRAUS M H ET AL: "Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors.", PNAS USA, vol. 86, no. 23, December 1989 (1989-12-01), pages 9193 - 9197 & HELLYER N J ET AL: "Cloning of the rat ErbB3 cDNA and characterization of the recombinant protein", GENE, vol. 165, no. 2, 20 November 1995 (1995-11-20), pages 279 - 284, XP004043156
  • [A] VADLAMUDI R K ET AL: "Heregulin and HER2 signaling selectively activates c-Src phosphorylation at tyrosine 215", FEBS LETT, vol. 543, no. 1-3, 22 May 2003 (2003-05-22), pages 76 - 80, XP004425037
  • [A] CELL SIGNALING TECHNOLOGY: "Phospho-PLCgamma1 (Tyr783) Antibody", INTERNET ARTICLE, 22 July 2004 (2004-07-22), pages 1 - 5, XP002334235, Retrieved from the Internet <URL:http://www.cellsignal.com/pdf/2821.pdf> [retrieved on 20050630]
  • [A] UPSTATE: "Antibodies for Phosphorylation & Beyond", INTERNET ARTICLE, June 2004 (2004-06-01), pages 1 - 16, XP002334236, Retrieved from the Internet <URL:http://www.upstate.com/img/pdf/antibodies_phos.pdf> [retrieved on 20050630]
  • [X] OLAYIOYE M: "The Erbbeta Signaling Network: Receptor Heterodimerization in Development and Cancer", EMBO J, vol. 19, no. 13, January 2000 (2000-01-01), pages 3159 - 3167, XP002985171
  • [X] VIJAPURKAR U ET AL: "Roles of mitogen-activated protein kinase and phosphoinositide 3'-kinase in ErbB2/ErbB3 coreceptor-mediated heregulin signaling.", EXP CELL RES, vol. 284, no. 2, 1 April 2003 (2003-04-01), pages 291 - 302, XP002467289
  • [A] KIM H H ET AL: "Epidermal growth factor-dependent association of phosphatidylinositol 3-kinase with the erbB3 gene product.", J BIOL CHEM, vol. 269, no. 40, 7 October 1994 (1994-10-07), pages 24747 - 24755, XP002467290
  • [A] ANONYMOUS: "Signaller: Advances in Signal Transduction Research", INTERNET ARTICLE, 11 July 2004 (2004-07-11), XP002467291, Retrieved from the Internet <URL:http://www.genesearch.com.au/downloads/Signaller2.pdf> [retrieved on 20080201]
  • [A] DATABASE UniProt [online] 1 May 1991 (1991-05-01), "Receptor tyrosine-protein kinase erbB-3 precursor (EC <A HREF="http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?[enzyme-ECNumber:2.7.10.1]+-e">2.7.10.1</A>) (c- erbB3) (Tyrosine kinase-type cell surface receptor HER3).", XP002467292, retrieved from EBI accession no. UNIPROT:P21860 Database accession no. P21860
  • [A] DATABASE UniProt [online] 15 December 1998 (1998-12-15), "Receptor tyrosine-protein kinase erbB-3 precursor (EC <A HREF="http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?[enzyme-ECNumber:2.7.10.1]+-e">2.7.10.1</A>) (c- erbB3).", XP002467293, retrieved from EBI accession no. UNIPROT:Q62799 Database accession no. Q62799
  • See references of WO 2006068640A1

Citation (examination)

VIJAPURKAR U. ET AL: "Mutation of a Shc binding site tyrosine residue in ErbB3/HER3 blocks heregulin-dependent activation of mitogen-activated protein kinase", J BIOL CHEM, vol. 273, no. 33, 14 August 1998 (1998-08-14), pages 20996 - 21002, XP001074635

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR

DOCDB simple family (publication)

WO 2006068640 A1 20060629; CA 2601700 A1 20060629; EP 1841882 A1 20071010

DOCDB simple family (application)

US 2004042940 W 20041221; CA 2601700 A 20041221; EP 04815061 A 20041221