Global Patent Index - EP 1846564 A2

EP 1846564 A2 2007-10-24 - METHOD FOR THE ENZYMATIC PRODUCTION OF ALPHA-KETOBUTYRATE

Title (en)

METHOD FOR THE ENZYMATIC PRODUCTION OF ALPHA-KETOBUTYRATE

Title (de)

VERFAHREN ZUR ENZYMATISCHEN HERSTELLUNG VON ALPHA-KETOBUTYRAT

Title (fr)

PROCEDE DESTINE A LA PRODUCTION ENZYMATIQUE D'ALPHA-CETOBUTYRATE

Publication

EP 1846564 A2 (EN)

Application

EP 06708069 A

Priority

  • EP 2006050722 W
  • US 65013605 P

Abstract (en)

[origin: WO2006082252A2] This invention relates to a process for the enzymatic production of a-ketobutyrate and its derivatives, in particular isoleucine. In the described process a-ketobutyrate is obtained from activated homoserine by ?-elimination. Homoserine is activated to either acylhomoserine or phosphohomoserine using homoserinetransacylase and homoserine kinase, respectively. Subsequently phosphohomoserine and/or acylhomoserine is/are transformed into phosphate or/and acylate, ammonia and a-ketobutyrate through the ?-eliminase activity of cystathionine- ?-synthase and/or acylhomoserine sulfliydrylase and/or phosphohomoserine sulfhydrylase. This reaction is favored by (i) reducing intracellular concentrations of H2S or cysteine, (ii) reducing the access of these two sulfur containing substrates to the enzyme cystathionine-?- synthase and/or acylhomoserine sulfhydrylase and/or phosphohomoserine sulfliydrylase or (iii) by using cystathionine-?-synthase and/or acylhomoserine sulfliydrylase and/or phosphohomoserine sulfhydrylase with high ?-elimination and low cystathionine-?-synthase and/or low acylhomoserine sulfliydrylase and/or low phosphohomoserine sulfhydrylase activity. At least one of these measures is applied in microorganisms, in particular coryneform bacteria and enterobacteria, in which as a consequence a-ketobutyrate and/or its derivatives can be produced in high quantities.

IPC 8 full level (invention and additional information)

C12P 13/06 (2006.01); C12P 7/42 (2006.01)

CPC (invention and additional information)

C12P 13/06 (2013.01); C12P 7/42 (2013.01)

Citation (search report)

See references of WO 2006082252A3

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

EPO simple patent family

WO 2006082252 A2 20060810; WO 2006082252 A3 20061207; EP 1846564 A2 20071024

INPADOC legal status


2012-10-17 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20120418

2008-05-14 [DAX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT (TO ANY COUNTRY) (DELETED)

2008-03-12 [17Q] FIRST EXAMINATION REPORT

- Effective date: 20080204

2007-10-24 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20070801

2007-10-24 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A2

- Designated State(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR