EP 1846564 A2 20071024 - METHOD FOR THE ENZYMATIC PRODUCTION OF ALPHA-KETOBUTYRATE
Title (en)
METHOD FOR THE ENZYMATIC PRODUCTION OF ALPHA-KETOBUTYRATE
Title (de)
VERFAHREN ZUR ENZYMATISCHEN HERSTELLUNG VON ALPHA-KETOBUTYRAT
Title (fr)
PROCEDE DESTINE A LA PRODUCTION ENZYMATIQUE D'ALPHA-CETOBUTYRATE
Publication
Application
Priority
- EP 2006050722 W 20060207
- US 65013605 P 20050207
Abstract (en)
[origin: WO2006082252A2] This invention relates to a process for the enzymatic production of a-ketobutyrate and its derivatives, in particular isoleucine. In the described process a-ketobutyrate is obtained from activated homoserine by ?-elimination. Homoserine is activated to either acylhomoserine or phosphohomoserine using homoserinetransacylase and homoserine kinase, respectively. Subsequently phosphohomoserine and/or acylhomoserine is/are transformed into phosphate or/and acylate, ammonia and a-ketobutyrate through the ?-eliminase activity of cystathionine- ?-synthase and/or acylhomoserine sulfliydrylase and/or phosphohomoserine sulfhydrylase. This reaction is favored by (i) reducing intracellular concentrations of H2S or cysteine, (ii) reducing the access of these two sulfur containing substrates to the enzyme cystathionine-?- synthase and/or acylhomoserine sulfhydrylase and/or phosphohomoserine sulfliydrylase or (iii) by using cystathionine-?-synthase and/or acylhomoserine sulfliydrylase and/or phosphohomoserine sulfhydrylase with high ?-elimination and low cystathionine-?-synthase and/or low acylhomoserine sulfliydrylase and/or low phosphohomoserine sulfhydrylase activity. At least one of these measures is applied in microorganisms, in particular coryneform bacteria and enterobacteria, in which as a consequence a-ketobutyrate and/or its derivatives can be produced in high quantities.
IPC 8 full level
C12P 7/42 (2006.01); C12P 13/06 (2006.01)
CPC (source: EP)
C12P 7/42 (2013.01); C12P 13/06 (2013.01)
Citation (search report)
See references of WO 2006082252A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2006082252 A2 20060810; WO 2006082252 A3 20061207; EP 1846564 A2 20071024
DOCDB simple family (application)
EP 2006050722 W 20060207; EP 06708069 A 20060207