EP 1883707 B1 20140409 - ISOTHERMAL STRAND DISPLACEMENT AMPLIFICATION USING PRIMERS CONTAINING A NON-REGULAR BASE
Title (en)
ISOTHERMAL STRAND DISPLACEMENT AMPLIFICATION USING PRIMERS CONTAINING A NON-REGULAR BASE
Title (de)
ISOTHERMISCHE STRANGVERDRÄNGUNGSAMPLIFIKATION UNTER VERWENDUNG VON EINE NICHTREGULÄRE BASE ENTHALTENDEN PRIMERN
Title (fr)
AMPLIFICATION PAR DEPLACEMENT DE BRIN ISOTHERME UTILISANT DES AMORCES CONTENANT UNE BASE NON REGULIERE
Publication
Application
Priority
- AU 2006000698 W 20060525
- US 68569705 P 20050526
Abstract (en)
[origin: WO2006125267A1] The invention is directed to a method for isothermal DNA amplification comprising providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing a non-regular base, a second primer at least partially complementary to a region of DNA and containing a non- regular base, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises a non-regular base in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near the non-regular base; and amplifying the DNA substantially without thermal cycling.
IPC 8 full level
C12N 15/11 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6865 (2013.01 - EP US)
Citation (examination)
- WO 03008642 A2 20030130 - KECK GRADUATE INST [US], et al
- EP 0497272 A1 19920805 - BECTON DICKINSON CO [US]
- EP 1312682 A1 20030521 - TAKARA BIO INC [JP]
- CASE-GREEN S C ET AL: "STUDIES ON THE BASE PAIRING PROPERTIES OF DEOXYNOSINE BY SOLID PHASE HYBRIDISATION TO OLIGONUCLEOTIDES", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 22, no. 2, 25 January 1994 (1994-01-25), pages 131 - 136, XP002024256, ISSN: 0305-1048
- HUANG J ET AL: "Multiple cleavage activities of endonuclease V from Thermotoga maritima: recognition and strand nicking mechanism", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 40, no. 30, 31 July 2001 (2001-07-31), pages 8738 - 8748, XP002441544, ISSN: 0006-2960, DOI: 10.1021/BI010183H
- FENG H ET AL: "Active site plasticity of endonuclease V from salmonella typhimurium", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 44, no. 2, 1 January 2005 (2005-01-01), pages 675 - 683, XP003021439, ISSN: 0006-2960, DOI: 10.1021/BI048752J
- LIU JIANG ET AL: "A deoxyinosine specific endonuclease from hyperthermophile, Archaeoglobus fulgidus: A homolog of Escherichia coli endonuclease V", MUTATION RESEARCH. DNA REPAIR, ELSEVIER, AMSTERDAM, NL, vol. 461, no. 3, 9 November 2000 (2000-11-09), pages 169 - 177, XP009086370, ISSN: 0921-8777, DOI: 10.1016/S0921-8777(00)00054-9
- TERRANCE WALKER G: "ISOTHERMAL IN VITRO AMPLIFICATION OF DNA BY A RESTRICTION ENZYME/ DNA POLYMERASE SYSTEM", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 89, no. 1, 1 January 1992 (1992-01-01), pages 392 - 396, XP000368694, ISSN: 0027-8424
- AMADIO M ET AL: "Strand displacement amplification for the diagnosis of chlamydia and gonorrhea in North Queensland", INTERNATIONAL ASM (AMERICAN SOCIETY FOR MICROBIOLOGY) CONFERENCE, XX, XX, 1 January 2000 (2000-01-01), pages 1 - 2, XP003021440
- "Endonuclease V", Retrieved from the Internet <URL:www.neb.com/nebecomm/products/productsM0305.asp> [retrieved on 20130114]
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2006125267 A1 20061130; AU 2006251866 A1 20061130; AU 2006251866 B2 20071129; CA 2609218 A1 20061130; CA 2609218 C 20161011; CN 101203618 A 20080618; CN 101203618 B 20130313; EP 1883707 A1 20080206; EP 1883707 A4 20090805; EP 1883707 B1 20140409; JP 2008541705 A 20081127; US 2010221785 A1 20100902; US 8431347 B2 20130430
DOCDB simple family (application)
AU 2006000698 W 20060525; AU 2006251866 A 20060525; CA 2609218 A 20060525; CN 200680018325 A 20060525; EP 06741118 A 20060525; JP 2008512651 A 20060525; US 91944306 A 20060525