EP 1891227 A2 20080227 - METHOD FOR QUANTIFYING LIVING COLIFORM MICROORGANISMS IN A WATER SAMPLE
Title (en)
METHOD FOR QUANTIFYING LIVING COLIFORM MICROORGANISMS IN A WATER SAMPLE
Title (de)
VERFAHREN ZUR QUANTIFIZIERUNG LEBENDER COLIFÖRMIGER MIKROORGANISMEN IN EINER WASSERPROBE
Title (fr)
PROCEDE DE QUANTIFICATION RAPIDE DES MICRO-ORGANISMES COLIFORMES VIVANTS DANS UN ECHANTILLON D'EAU
Publication
Application
Priority
- FR 2006050497 W 20060601
- FR 0505618 A 20050603
Abstract (en)
[origin: WO2006129038A2] The invention concerns a method for rapidly quantifying living coliform microorganisms in a water sample which consists in contacting the microorganisms with a fluorogenic substrate which is hydrolyzed in methylumbelliferone by an enzyme released by the microorganisms. The methylumbelliferone is detected by the fluorescence it emits, the speed of variation of said fluorescence being measured so as to determine the concentration of coliform microorganisms in the original sample based on the calculated speed of variation and on a correlation scheme. Typically, the method consists in carrying out a preliminary step of treating, in particular by pre-filtering, the sample to remove at least part of the particles suspended in the water, thereby allowing a quantification quite comparable to that of the method normalized by microplate. The invention is applicable to swimming water for detecting Escherichia Coli.
IPC 8 full level
C12Q 1/04 (2006.01); C12Q 1/06 (2006.01); C12Q 1/10 (2006.01); G01N 33/18 (2006.01)
CPC (source: EP US)
C12Q 1/04 (2013.01 - EP US); C12Q 1/06 (2013.01 - EP US); C12Q 1/10 (2013.01 - EP US)
Citation (search report)
See references of WO 2006129038A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2006129038 A2 20061207; WO 2006129038 A3 20070419; AU 2006253993 A1 20061207; CA 2611300 A1 20061207; EP 1891227 A2 20080227; FR 2886651 A1 20061208; FR 2886651 B1 20070921; US 2009221013 A1 20090903
DOCDB simple family (application)
FR 2006050497 W 20060601; AU 2006253993 A 20060601; CA 2611300 A 20060601; EP 06778910 A 20060601; FR 0505618 A 20050603; US 91632606 A 20060601