EP 1930727 B1 20130417 - METHOD OF QUANTIFYING THE G PROTEIN-COUPLED RECEPTOR (GPCR)/G PROTEIN COUPLING USING A CELL MEMBRANE ARRAY
Title (en)
METHOD OF QUANTIFYING THE G PROTEIN-COUPLED RECEPTOR (GPCR)/G PROTEIN COUPLING USING A CELL MEMBRANE ARRAY
Title (de)
VERFAHREN ZUR QUANTIFIZIERUNG DER KOPPLUNG G-PROTEIN-GEKOPPELTER REZEPTOR (GPCR)/G-PROTEIN UNTER VERWENDUNG EINES ZELLMEMBRAN-ARRAYS
Title (fr)
METHODE DE QUANTIFICATION DE LA LIAISON D'UN RECEPTEUR COUPLE A UNE PROTEINE G (GPCR) AU MOYEN D'UN RESEAU DE MEMBRANES CELLULAIRES
Publication
Application
Priority
- ES 2006000445 W 20060728
- ES 200501859 A 20050728
Abstract (en)
[origin: EP1930727A2] The invention relates to a method for quantifying G protein-coupled receptor (GPCR)-G protein binding by means of using a cell membrane array, which comprises (i) putting an unlabeled candidate compound in contact with a cell membrane array in the presence of labeled GTP or of a labeled, non-hydrolyzable analog thereof, in conditions allowing the interaction between said compound and said GPCR present in said cell membranes, and between said labeled GTP or analog thereof and said G protein present in said membranes; (ii) washing; and (iii) quantifying the signal obtained due to the binding of the labeled GTP (or analog) to said G protein. It is applicable in the analysis of the interaction between compounds and cell membrane receptor proteins and of the intracellular signaling mechanisms triggering this interaction mechanism mediated by said compounds.
IPC 8 full level
G01N 33/566 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP ES US)
G01N 33/5735 (2013.01 - EP ES US); G01N 33/74 (2013.01 - EP ES US); G01N 33/9406 (2013.01 - EP ES US); G01N 2333/4719 (2013.01 - EP US); G01N 2333/726 (2013.01 - EP US); G01N 2500/02 (2013.01 - EP US)
Citation (examination)
- EP 2048534 A1 20090415 - UNIV PAIS VASCO [ES], et al
- GILLE ANDREAS ET AL: "Low-affinity interactions of BODIPY-FL-GTPgammaS and BODIPY-FL-GppNHp with G(i)- and G(s)-proteins.", NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY SEP 2003 LNKD- PUBMED:12920505, vol. 368, no. 3, September 2003 (2003-09-01), pages 210 - 215, ISSN: 0028-1298
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
EP 1930727 A2 20080611; EP 1930727 B1 20130417; ES 2322727 A1 20090625; ES 2322727 B1 20100422; US 2008318799 A1 20081225; US 8017346 B2 20110913; WO 2007012688 A2 20070201; WO 2007012688 A3 20070412
DOCDB simple family (application)
EP 06807893 A 20060728; ES 200501859 A 20050728; ES 2006000445 W 20060728; US 99696506 A 20060728