EP 1941281 A1 20080709 - METHOD FOR SCREENING A PROTEASOME ACTIVITY MODULATING AGENT AND MEANS FOR CARRYING OUT SAID METHOD
Title (en)
METHOD FOR SCREENING A PROTEASOME ACTIVITY MODULATING AGENT AND MEANS FOR CARRYING OUT SAID METHOD
Title (de)
VERFAHREN ZUM SCREENING EINES PROTEASOMAKTIVITÄTSMODULIERENDEN WIRKSTOFFES UND MITTEL ZUR AUSFÜHRUNG DIESES VERFAHRENS
Title (fr)
PROCEDE DE CRIBLAGE D'AGENTS MODULANT L'ACTIVITE DU PROTEASOME ET MOYENS DESTINES A LA MISE EN OEUVRE DUDIT PROCEDE
Publication
Application
Priority
- FR 2006051062 W 20061019
- FR 0553183 A 20051019
Abstract (en)
[origin: WO2007045799A1] The invention relates to an in cellulo method for screening a proteasome activity modulating agent consisting a) in bringing a testable candidate agent into contact with recombinant yeast cells expressing in the core thereof a fusion protein comprising (i) a p21 polypeptide selected from p21<SUP>WAF1/Cip1 </SUP>and p21[6KR]<SUP>WAF1/Cip1</SUP> polypeptides and (ii) at least one type of detectable protein, b) in quantifying said first detectable protein in the yeast cells at the end of at least one predetermined time interval after bringing the candidate agent into contact with the yeast cells, and c) in comparing the value obtained at the stage (b) with a control value obtained when the stage (a) is performed in the absence of the candidate agent.
IPC 8 full level
G01N 33/68 (2006.01); C12N 1/16 (2006.01); C12N 15/81 (2006.01); G01N 33/58 (2006.01)
CPC (source: EP US)
C07K 14/4738 (2013.01 - EP US); C12N 15/81 (2013.01 - EP US); C12Q 1/025 (2013.01 - EP US); G01N 33/6872 (2013.01 - EP US); G01N 2500/00 (2013.01 - EP US)
Citation (search report)
See references of WO 2007045799A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
FR 2892199 A1 20070420; FR 2892199 B1 20080201; CA 2626600 A1 20070426; EP 1941281 A1 20080709; JP 2009512432 A 20090326; US 2010216160 A1 20100826; WO 2007045799 A1 20070426
DOCDB simple family (application)
FR 0553183 A 20051019; CA 2626600 A 20061019; EP 06820318 A 20061019; FR 2006051062 W 20061019; JP 2008536102 A 20061019; US 9086706 A 20061019