EP 1944368 A1 20080716 - Nucleic acid isolation method by heating on magnetic support
Title (en)
Nucleic acid isolation method by heating on magnetic support
Title (de)
Verfahren zur Isolierung von Nukleinsäure durch Erhitzung auf einem magnetischen Träger
Title (fr)
Procédé d'isolation d'acides nucléiques par chauffage sur support magnétique
Publication
Application
Priority
JP 2007006409 A 20070115
Abstract (en)
A method for isolating a nucleic acid from a cell-containing sample by using a magnet with a removable cover attached thereto and a plurality of containers arranged on a table, the plurality of containers having (1) a first container for mixing a cell-containing sample and magnetic beads to which cells can be adhered, (2) a cleaning tank for cleaning the magnetic beads with cells adhering thereto, and (3) a second container for heating the cleaned magnetic beads, the method includes: mixing a liquid including cell-containing sample and magnetic beads in the cell-containing sample in the first container so as to adhere cells in the cell-containing sample to magnetic beads; taking out the magnetic beads from the first container and cleaning the magnetic beads; and suspending the magnetic beads in a re-suspension buffer in the second container and heating the second container at 80 to 120 °C for 20 to 300.
IPC 8 full level
C12N 15/10 (2006.01)
CPC (source: EP US)
C12N 15/1013 (2013.01 - EP US)
Citation (applicant)
- JP 2007006409 A 20070111 - MATSUSHITA ELECTRIC WORKS LTD
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Citation (search report)
- [X] EP 1655366 A2 20060510 - SAMSUNG ELECTRONICS CO LTD [KR]
- [X] US 6255478 B1 20010703 - KOMAI SHIGERU [JP], et al
- [X] EP 0933132 A2 19990804 - TOYO BOSEKI [JP]
Citation (examination)
US 2003183580 A1 20031002 - SCHAAF WILLIAM R [US], et al
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA MK RS
DOCDB simple family (publication)
EP 1944368 A1 20080716; CN 101255417 A 20080903; JP 2008167722 A 20080724; US 2008171337 A1 20080717
DOCDB simple family (application)
EP 08150067 A 20080107; CN 200810096647 A 20080111; JP 2007006409 A 20070115; US 1303308 A 20080111