EP 1947196 A1 20080723 - METHOD OF AMPLIFYING MULTIPLE NUCLEIC ACID SEQUENCES FOR DIFFERENTIATION
Title (en)
METHOD OF AMPLIFYING MULTIPLE NUCLEIC ACID SEQUENCES FOR DIFFERENTIATION
Title (de)
VERFAHREN ZUR AMPLIFIKATION MEHRERER NUKLEINSÄURESEQUENZEN ZUR DIFFERENZIERUNG
Title (fr)
PROCÉDÉ D'AMPLIFICATION DE PLUSIEURS SÉQUENCES D'ACIDES NUCLÉIQUES EN VUE DE LEUR DIFFÉRENCIATION
Publication
Application
Priority
- JP 2006322304 W 20061108
- JP 2005323726 A 20051108
Abstract (en)
The invention provides a method with fewer distribution of the amount of amplification among plural tags when the plural tags are used for multiplex detection using the tag. A method for amplifying plural nucleic acid fragments, the nucleic acid fragment including one nucleic acid sequence for discrimination selected from a first group consisting of first to n-th nucleic acid sequences for discrimination and one nucleic acid sequence for amplification selected from a second group consisting of first to m-th nucleic acid sequences for amplification, the method including amplifying a mixture of nucleic acid fragments containing plural kinds of the nucleic acid fragments under a condition capable of amplifying the nucleic acids using, as primers, at least a part of the sequences of all the nucleic acid sequences for discrimination that may exist in the mixture of the nucleic acid fragments, and sequences complementary to at least a part of the sequences of the nucleic acid sequences for amplification or sequences at the same strand side as the nucleic acid sequences for amplification, wherein n is an integer of 2 or more, the first to n-th nucleic acid sequences for discrimination contained in the first group have different nucleotide sequences from one another, m is an integer from 1 or more to n or less, and the first to m-th nucleic acid sequences for amplification contained in the second group have different nucleotide sequences from one another.
IPC 8 full level
C12Q 1/68 (2006.01); C12N 15/09 (2006.01)
CPC (source: EP US)
C12Q 1/6853 (2013.01 - EP US)
C-Set (source: EP US)
Designated contracting state (EPC)
AT DE DK FR GB SE
DOCDB simple family (publication)
EP 1947196 A1 20080723; EP 1947196 A4 20090909; CN 101268201 A 20080917; JP WO2007055255 A1 20090430; US 2009061440 A1 20090305; WO 2007055255 A1 20070518
DOCDB simple family (application)
EP 06823208 A 20061108; CN 200680034738 A 20061108; JP 2006322304 W 20061108; JP 2007544166 A 20061108; US 11672808 A 20080507