EP 1948795 A1 20080730 - METHOD FOR SELECTIVELY AND REVERSIBLY ADSORBING NUCLEIC ACIDS ON A CARRIER MATERIAL
Title (en)
METHOD FOR SELECTIVELY AND REVERSIBLY ADSORBING NUCLEIC ACIDS ON A CARRIER MATERIAL
Title (de)
VERFAHREN ZUR SELEKTIVEN, REVERSIBLEN ADSORPTION VON NUKLEINSÄUREN AN EIN TRÄGERMATERIAL
Title (fr)
PROCEDE D'ADSORPTION SELECTIVE ET REVERSIBLE D'ACIDES NUCLEIQUES SUR UN SUPPORT
Publication
Application
Priority
- EP 2006068179 W 20061107
- EP 05024207 A 20051107
- EP 06819298 A 20061107
Abstract (en)
[origin: EP1783220A1] A method for selective, reversible adsorption of nucleic acids on a substrate, comprises contacting nucleic-acid mixture with the substrate, in which the nucleic acids are adsorbed, purifying nucleic acids with an ethanol/isopropanol or a precipitating agent, and removing the nucleic acids bounded on the substrate by an aqueous solution exhibiting 1 mole of salt content, where the nucleic-acid mixture exhibits 1 mole of kosmotrope salts content, the kosmotrope is a sulfate, citrate or phosphate, and the substrate is a membrane and a filter. The method for selective, reversible adsorption of nucleic acids on a substrate, comprises contacting nucleic-acid mixture with the substrate, in which the nucleic acids are adsorbed, purifying nucleic acids with an ethanol/isopropanol or a precipitating agent, and removing the nucleic acids bounded on the substrate by an aqueous solution exhibiting 1 mole of salt content. The nucleic-acid mixture exhibits 1 mole of kosmotrope salts content. The kosmotrope is a sulfate, citrate or phosphate. The substrate is a membrane and a filter. The filter is a nitrocellulose filter or a hydrophilized nylon filter. The adsorbent consists of silica surfaces. In the contacting process, more than 1 molar solution of a kosmotrope salt is used. The purification is carried out after the aqueous two-phase separation of nucleic acids.
IPC 8 full level
C12N 15/10 (2006.01)
CPC (source: EP US)
C12N 15/1006 (2013.01 - EP US)
Citation (search report)
See references of WO 2007051864A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
EP 1783220 A1 20070509; EP 1948795 A1 20080730; US 2010036109 A1 20100211; WO 2007051864 A1 20070510
DOCDB simple family (application)
EP 05024207 A 20051107; EP 06819298 A 20061107; EP 2006068179 W 20061107; US 8466706 A 20061107