EP 1957649 A1 20080820 - PROCESS FOR THE PRODUCTION, IN PROKARYOTES, OF ACTIVE, STABLE TRANSPOSASES OF MARINER MOBILE GENETIC ELEMENTS
Title (en)
PROCESS FOR THE PRODUCTION, IN PROKARYOTES, OF ACTIVE, STABLE TRANSPOSASES OF MARINER MOBILE GENETIC ELEMENTS
Title (de)
VERFAHREN ZUR PRODUKTION AKTIVER, STABILER TRANSPOSASEN MOBILER GENETISCHER ELEMENTE AUS MARINER IN PROKARYONTEN
Title (fr)
PROCEDE DE PRODUCTION CHEZ LES PROCARYOTES DE TRANSPOSASES ACTIVES ET STABLES D'ELEMENTS GENETIQUES MOBILES MARINER
Publication
Application
Priority
- EP 2006068853 W 20061123
- FR 0512180 A 20051130
Abstract (en)
[origin: FR2893951A1] Producing a transposase of a mauritiana subfamily mariner transposon in a prokaryotic host cell comprises cloning the transposase coding sequence (S1) into an expression vector, cloning a cAMP-dependent protein kinase catalytic subunit coding sequence (S2) into an expression vector and transforming the host cell with the vectors. Independent claims are also included for: (1) transposase produced as above; (2) prokaryotic host cell comprising S1 and S2; (3) expression vector comprising S1 and S2; (4) kit for insertional, sequencing or cloning mutagenesis comprising a transposase as above.
IPC 8 full level
C12N 15/55 (2006.01); A61K 38/46 (2006.01); C12N 9/22 (2006.01)
CPC (source: EP US)
C12N 9/22 (2013.01 - EP US); A61K 38/00 (2013.01 - EP US)
Citation (search report)
See references of WO 2007063033A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
FR 2893951 A1 20070601; FR 2893951 B1 20080229; CA 2631400 A1 20070607; EP 1957649 A1 20080820; JP 2009517059 A 20090430; US 2009311743 A1 20091217; WO 2007063033 A1 20070607
DOCDB simple family (application)
FR 0512180 A 20051130; CA 2631400 A 20061123; EP 06830106 A 20061123; EP 2006068853 W 20061123; JP 2008542736 A 20061123; US 8543506 A 20061123