EP 1976998 A4 20100825 - METHOD FOR RAPID DETECTION AND EVALUATION OF CULTURED CELL GROWTH
Title (en)
METHOD FOR RAPID DETECTION AND EVALUATION OF CULTURED CELL GROWTH
Title (de)
VERFAHREN ZUR SCHNELLEN ERKENNUNG UND BEURTEILUNG VON KULTIVIERTEM ZELLWACHSTUM
Title (fr)
PROCEDE POUR REALISER UNE DETECTION ET UNE EVALUATION RAPIDES DE LA CROISSANCE DE CELLULES DE CULTURE
Publication
Application
Priority
- US 2007001410 W 20070118
- US 33423606 A 20060118
Abstract (en)
[origin: US2007166780A1] Provided is a method and system for the rapid and accurate detection of growth and metabolism of a cellular microorganism in a population of microorganisms in a non-liquid, culture medium. Further provided is a gelled culture medium containing a non-toxic, water-soluble, phosphorescent compound which measures oxygen content (partial pressure) of an microorganism also contained therein, by oxygen-dependent quenching of phosphorescence; or the gel contains a fluorescent pH indicator that demonstrates growth of the microorganism by pH-dependent intensity change or wavelength shift in the emission spectrum. Further provided is a system and method for killing undesirable microorganisms or colonies in the culture medium without harming the surrounding microorganisms.
IPC 8 full level
C12Q 1/02 (2006.01)
CPC (source: EP US)
A61K 31/407 (2013.01 - EP US); A61K 31/555 (2013.01 - EP US); C12M 25/14 (2013.01 - EP US); C12M 41/36 (2013.01 - EP US); C12M 41/46 (2013.01 - EP US); G01N 33/84 (2013.01 - EP US)
Citation (search report)
- [X] US 6395506 B1 20020528 - PITNER J BRUCE [US], et al
- [X] WO 0126609 A2 20010419 - UNIV PENNSYLVANIA [US], et al
- [X] KWOK ET AL: "An optical biosensor for multi-sample determination of biochemical oxygen demand (BOD)", SENSORS AND ACTUATORS B, ELSEVIER SEQUOIA S.A., LAUSANNE, CH LNKD- DOI:10.1016/J.SNB.2005.02.007, vol. 110, no. 2, 14 October 2005 (2005-10-14), pages 289 - 298, XP005023955, ISSN: 0925-4005
- [X] DAI Y-J ET AL: "COMPARISON OF BOD OPTICAL FIBER BIOSENSORS BASED ON DIFFERENT MICROORGANISMS IMMOBILIZED IN ORMOSIL MATRIXES", INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, LONDON, GB, vol. 84, no. 8, 15 July 2004 (2004-07-15), pages 607 - 617, XP009068500
- [X] OMAR SANAA H: "Oxygen diffusion through gels employed for immobilization. 1. In the absence of microorganisms", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 40, no. 1, 1 January 1993 (1993-01-01), pages 1 - 6, XP009135414, ISSN: 0175-7598
- See references of WO 2007084655A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
US 2007166780 A1 20070719; US 7575890 B2 20090818; AU 2007207450 A1 20070726; AU 2007207450 B2 20120607; CA 2637840 A1 20070726; EP 1976998 A2 20081008; EP 1976998 A4 20100825; JP 2009523447 A 20090625; US 2009280522 A1 20091112; WO 2007084655 A2 20070726; WO 2007084655 A3 20071101
DOCDB simple family (application)
US 33423606 A 20060118; AU 2007207450 A 20070118; CA 2637840 A 20070118; EP 07716791 A 20070118; JP 2008551408 A 20070118; US 2007001410 W 20070118; US 50434609 A 20090716