EP 1979493 A1 20081015 - METHODS FOR MEASURING KINASE ACTIVITY
Title (en)
METHODS FOR MEASURING KINASE ACTIVITY
Title (de)
VERFAHREN ZUM MESSEN DER KINASEAKTIVITÄT
Title (fr)
PROCEDES DE MESURE DE L'ACTIVITE D'UNE KINASE
Publication
Application
Priority
- US 2007060729 W 20070118
- US 75991906 P 20060118
- US 81943206 P 20060707
Abstract (en)
[origin: WO2007084968A1] The present invention relates to methods for detecting and/or measuring the activity of a specific kinase, with the methods comprising contacting one or more kinases with a binding agent to isolate a specific kinase of interest. The isolated kinase is then contacted with a kinase activity sensor, where the kinase activity sensor is comprised of a kinase recognition motif that is capable of being recognized by the isolated kinase, and at least one phosphorylation site. The isolated kinase phosphorylates the amino acid target of the kinase activity sensor and levels of the phosphorylated target amino acid can then be quantified.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/485 (2013.01); G01N 33/573 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2007084968 A1 20070726; EP 1979493 A1 20081015; EP 1979493 A4 20090311; JP 2009523452 A 20090625
DOCDB simple family (application)
US 2007060729 W 20070118; EP 07717310 A 20070118; JP 2008551537 A 20070118