EP 1996931 B1 20131127 - BLOOD CELL SORTING METHODS AND SYSTEMS
Title (en)
BLOOD CELL SORTING METHODS AND SYSTEMS
Title (de)
BLUTZELLEN-SORTIERUNGSVERFAHREN UND -SYSTEME
Title (fr)
PROCEDES ET SYSTEMES DE TRI DE GLOBULES SANGUINS
Publication
Application
Priority
- US 2006049437 W 20061228
- US 75442605 P 20051228
Abstract (en)
[origin: WO2007079149A2] The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
IPC 8 full level
G01N 33/00 (2006.01); G01N 33/48 (2006.01)
CPC (source: EP US)
C12N 5/0087 (2013.01 - EP US); C12N 5/0634 (2013.01 - EP US); C12N 13/00 (2013.01 - EP US); G01N 33/491 (2013.01 - EP US); Y10T 436/10 (2015.01 - EP US); Y10T 436/101666 (2015.01 - EP US); Y10T 436/108331 (2015.01 - EP US); Y10T 436/25 (2015.01 - EP US); Y10T 436/25125 (2015.01 - EP US); Y10T 436/25375 (2015.01 - EP US); Y10T 436/255 (2015.01 - EP US)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2007079149 A2 20070712; WO 2007079149 A3 20070913; DK 1996931 T3 20140217; EP 1996931 A2 20081203; EP 1996931 A4 20090819; EP 1996931 B1 20131127; US 2007163963 A1 20070719; US 2011111476 A1 20110512; US 2012295246 A1 20121122; US 2015111276 A1 20150423; US 2015322424 A1 20151112; US 7867765 B2 20110111; US 8187886 B2 20120529; US 8753888 B2 20140617; US 9410144 B2 20160809
DOCDB simple family (application)
US 2006049437 W 20061228; DK 06848249 T 20061228; EP 06848249 A 20061228; US 201213481237 A 20120525; US 201414291313 A 20140530; US 201514806112 A 20150722; US 64698506 A 20061228; US 98757511 A 20110110