Global Patent Index - EP 2004141 A2

EP 2004141 A2 20081224 - AN EFFICIENT METHOD FOR LOADING AMPHOTERIC LIPOSOMES WITH NUCLEIC ACID ACTIVE SUBSTANCES

Title (en)

AN EFFICIENT METHOD FOR LOADING AMPHOTERIC LIPOSOMES WITH NUCLEIC ACID ACTIVE SUBSTANCES

Title (de)

EFFIZIENTES VERFAHREN ZUM BELADEN VON AMPHOTEREN LIPOSOMEN MIT NUKLEINSÄURE-WIRKSTOFFEN

Title (fr)

PROCÉDÉ EFFICACE DE CHARGEMENT DE LIPOSOMES AMPHOTÈRES AVEC DES SUBSTANCES ACTIVES D'ACIDES NUCLÉIQUES

Publication

EP 2004141 A2 20081224 (EN)

Application

EP 07723327 A 20070316

Priority

  • EP 2007002349 W 20070316
  • DE 102006013121 A 20060317
  • EP 06113784 A 20060510
  • EP 2006009013 W 20060915
  • EP 06254821 A 20060915
  • US 52185706 A 20060915
  • US 58105406 A 20061013
  • EP 06255277 A 20061013
  • DE 102006054192 A 20061110
  • EP 07723327 A 20070316

Abstract (en)

[origin: WO2007107304A2] A method for preparing amphoteric liposomes loaded with a polyanionic active agent as cargo, characterised by admixing an aqueous solution of said polyanionic active agent and an alcoholic solution of one or more amphiphiles and buffering said admixture to an acidic pH, said one or more amphiphiles being susceptible of forming amphoteric liposomes at said acidic pH, thereby to form such amphoteric liposomes in suspension encapsulating said active agent under conditions such that said liposomes form aggregates, and thereafter treating said suspension to dissociate said aggregates. Also disclosed are nucleic acid loaded amphoteric liposomes produced in accordance with the method, wherein said nucleic acids are oligonucleotides and said liposomes are multilamellar.

IPC 8 full level

A61K 9/127 (2006.01); A61K 48/00 (2006.01); C12N 15/88 (2006.01)

CPC (source: EP)

A61K 9/1272 (2013.01); A61K 9/1277 (2013.01); A61K 48/00 (2013.01); C07H 21/00 (2013.01); C12N 15/88 (2013.01); Y02P 20/582 (2015.11)

Citation (search report)

See references of WO 2007107304A2

Citation (examination)

  • EP 1764091 A2 20070321 - NOVOSOM AG [DE]
  • WO 2007064857 A2 20070607 - PRONAI THERAPEUTICS INC [US], et al
  • WO 2006048329 A1 20060511 - NOVOSOM AG [DE], et al
  • WO 2005094783 A2 20051013 - NOVOSOM AG [DE], et al
  • ZUIDAM NICOLAAS J ET AL: "Electrostatic parameters of cationic liposomes commonly used for gene delivery as determined by 4-heptadecyl-7-hydroxycoumarin", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1329, no. 2, 23 October 1997 (1997-10-23), pages 211 - 222, ISSN: 0006-3002
  • TSUI F C ET AL: "THE INTRINSIC PK-A VALUES FOR PHOSPHATIDYLSERINE AND PHOSPHATIDYLETHANOLAMINE IN PHOSPHATIDYLCHOLINE HOST BILAYERS", BIOPHYSICAL JOURNAL, vol. 49, no. 2, 1986, pages 459 - 468, ISSN: 0006-3495
  • LOBO BRIAN A ET AL: "Thermodynamic analysis of binding and protonation in DOTAP/DOPE (1:1): DNA complexes using isothermal titration calorimetry.", BIOPHYSICAL CHEMISTRY, vol. 104, no. 1, 1 May 2003 (2003-05-01), pages 67 - 78, ISSN: 0301-4622

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

Designated extension state (EPC)

AL BA HR MK RS

DOCDB simple family (publication)

WO 2007107304 A2 20070927; WO 2007107304 A3 20080612; EP 2004141 A2 20081224

DOCDB simple family (application)

EP 2007002349 W 20070316; EP 07723327 A 20070316