EP 2017610 A4 20100203 - IONIZING METHOD AND DEVICE BY ELECTROSPRAY
Title (en)
IONIZING METHOD AND DEVICE BY ELECTROSPRAY
Title (de)
IONISIERUNGSVERFAHREN UND -VORRICHTUNG MIT ELEKTROSPRAY
Title (fr)
PROCEDE ET DISPOSITIF D'IONISIATION PAR ELECTRONEBULISATION
Publication
Application
Priority
- JP 2007059438 W 20070426
- JP 2006125398 A 20060428
- JP 2006135205 A 20060515
Abstract (en)
[origin: EP2017610A1] The invention enables measurement of a biosample without preprocessing, desorptive ionization of ions under the atmospheric pressure, and imaging at a spatial resolution of nm order. A sample object of measurement without preprocessing under the atmospheric pressure is, e.g., a biological tissue. An STM probe with an XYZ-axis drive piezoelectric element is vibrated vertically by a Z-direction drive at a frequency of several Hz to several kHz with amplitudes of several 10 µm to several mm, and brought into contact with the biosample at nm order depth to capture molecules of the biosample by the probe tip. A pulse high voltage is applied to the probe tip thereby realizing probe electrospray. The molecules of the sample captured by the probe tip are desorptively-ionized by Plasmon-assisted electrospray or infrared laser heating assist, and are mass-analyzed by orthogonal time-of-flight mass spectrometry or an ion trap mass spectrometer. The probe is scanned in the X- and Y-directions to produce repetitive vibration and the molecule image in the nm region is measured. In another mode, a probe is brought in contact with a droplet produced on the tip of the capillary connected to the exit of a liquid chromatograph, a liquid sample is captured, and electrospray is produced.
IPC 8 full level
G01N 30/72 (2006.01); G01N 27/62 (2006.01); H01J 49/04 (2006.01)
CPC (source: EP US)
B82Y 35/00 (2013.01 - US); G01N 27/623 (2021.01 - EP US); G01N 30/7266 (2013.01 - EP US); G01Q 30/02 (2013.01 - EP US); G01Q 60/16 (2013.01 - EP US); H01J 49/0004 (2013.01 - EP US); H01J 49/161 (2013.01 - EP US); H01J 49/165 (2013.01 - EP US); G01N 2001/028 (2013.01 - EP US)
Citation (search report)
- [XYI] US 5945678 A 19990831 - YANAGISAWA YUTARO [JP]
- [XYI] JP H0723796 A 19950127 - HITACHI LTD
- [XAI] WO 2004034057 A2 20040422 - BIOTROVE INC [US]
- [XY] WO 2004018104 A1 20040304 - BIOTROVE INC [US], et al
- [XAY] US 2004051037 A1 20040318 - TAYLOR ROD [CA], et al
- [Y] US 6551557 B1 20030422 - ROSE DON [US], et al
- [Y] WO 02082073 A2 20021017 - MDS INC DBA MDS SCIEX [CA], et al
- See references of WO 2007126141A1
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
EP 2017610 A1 20090121; EP 2017610 A4 20100203; EP 2017610 B1 20141001; JP 4862167 B2 20120125; JP WO2007126141 A1 20090917; US 2009140137 A1 20090604; US 7902499 B2 20110308; WO 2007126141 A1 20071108
DOCDB simple family (application)
EP 07742873 A 20070426; JP 2007059438 W 20070426; JP 2008513335 A 20070426; US 25987108 A 20081028