EP 2021497 A1 20090211 - MODIFIED MICROBIAL NUCLEIC ACID FOR USE IN DETECTION AND ANALYSIS OF MICROORGANISMS
Title (en)
MODIFIED MICROBIAL NUCLEIC ACID FOR USE IN DETECTION AND ANALYSIS OF MICROORGANISMS
Title (de)
MODIFIZIERTE MIKROBIELLE NUKLEINSÄURE ZUR VERWENDUNG BEIM NACHWEIS UND BEI DER ANALYSE VON MIKROORGANISMEN
Title (fr)
ACIDE NUCLÉIQUE MICROBIEN MODIFIÉ DESTINÉ À LA DÉTECTION ET À L'ANALYSE DE MICRO-ORGANISMES
Publication
Application
Priority
AU 2006000755 W 20060602
Abstract (en)
[origin: WO2007140506A1] The present invention provides derivatives or modified nucleic acid sequences of several microorganisms for use in the detection and analysis of said microorganisms. The derivative nucleic acids contain the bases adenosine (A), guanosine (G), T (thymine) and U (uracil or some other non-A, G or T base or base-like entity). Given that microbial nucleic acids do not contain methylated cytosine (C) or other C alterations, all C are converted to U. These sequences are amplified where the U in the derivative nucleic acid is replaced by a T, resulting in a modified sequence with the same number of total bases as the corresponding unmodified microbial nucleic acid sequence but made up of a combination of three bases only: A, G and T. As a consequence of this process the nucleic acids derived from the upper and lower strands of the original dsDNA are no longer complementary and the modified microbial sequences have reduced relative genomic complexity for use in detection and analysis of microorganisms.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/689 (2013.01)
Citation (search report)
See references of WO 2007140506A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA HR MK YU
DOCDB simple family (publication)
WO 2007140506 A1 20071213; WO 2007140506 A8 20100415; AU 2006344331 A1 20071213; CA 2654010 A1 20071213; CN 101522908 A 20090902; EP 2021497 A1 20090211; JP 2009538603 A 20091112
DOCDB simple family (application)
AU 2006000755 W 20060602; AU 2006344331 A 20060602; CA 2654010 A 20060602; CN 200680055508 A 20060602; EP 06741172 A 20060602; JP 2009512366 A 20060602