Global Patent Index - EP 2021497 A1

EP 2021497 A1 2009-02-11 - MODIFIED MICROBIAL NUCLEIC ACID FOR USE IN DETECTION AND ANALYSIS OF MICROORGANISMS

Title (en)

MODIFIED MICROBIAL NUCLEIC ACID FOR USE IN DETECTION AND ANALYSIS OF MICROORGANISMS

Title (de)

MODIFIZIERTE MIKROBIELLE NUKLEINSÄURE ZUR VERWENDUNG BEIM NACHWEIS UND BEI DER ANALYSE VON MIKROORGANISMEN

Title (fr)

ACIDE NUCLÉIQUE MICROBIEN MODIFIÉ DESTINÉ À LA DÉTECTION ET À L'ANALYSE DE MICRO-ORGANISMES

Publication

EP 2021497 A1 (EN)

Application

EP 06741172 A

Priority

AU 2006000755 W

Abstract (en)

[origin: WO2007140506A1] The present invention provides derivatives or modified nucleic acid sequences of several microorganisms for use in the detection and analysis of said microorganisms. The derivative nucleic acids contain the bases adenosine (A), guanosine (G), T (thymine) and U (uracil or some other non-A, G or T base or base-like entity). Given that microbial nucleic acids do not contain methylated cytosine (C) or other C alterations, all C are converted to U. These sequences are amplified where the U in the derivative nucleic acid is replaced by a T, resulting in a modified sequence with the same number of total bases as the corresponding unmodified microbial nucleic acid sequence but made up of a combination of three bases only: A, G and T. As a consequence of this process the nucleic acids derived from the upper and lower strands of the original dsDNA are no longer complementary and the modified microbial sequences have reduced relative genomic complexity for use in detection and analysis of microorganisms.

IPC 8 full level (invention and additional information)

C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/689 (2013.01)

Citation (search report)

See references of WO 2007140506A1

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

Designated extension state (EPC)

AL BA HR MK YU

EPO simple patent family

WO 2007140506 A1 20071213; WO 2007140506 A8 20100415; AU 2006344331 A1 20071213; CA 2654010 A1 20071213; CN 101522908 A 20090902; EP 2021497 A1 20090211; JP 2009538603 A 20091112

INPADOC legal status


2011-02-02 [18W] APPLICATION WITHDRAWN

- Effective date: 20101223

2009-02-11 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20081202

2009-02-11 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

2009-02-11 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO:

- Countries: AL BA HR MK YU